Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 270-232-7 | CAS number: 68413-60-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
96h LC50 208 mg/l for Pimephales promelas (Key study)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 208 mg/L
Additional information
Data are available for three short-term studies on fish (based on read-across, for justification see IUCLID section 13):
The key study was performed with sodium ferric EDTA (FeNH4 -EDTA) according to the "Standard methods for the examination of water and wastewater, 13th edition (American Public Health Association)" prior to GLP and was assigned reliability 2. This was nevertheless chosen as the key study because the substance tested was the most similar to EDTA-FeNH4(NH4)OH. This test revealed a 96h-LC50 for Pimphales promelas of 190 mg EDTA-FeNH4 per liter, which is equivalent to 208 mg EDTA-FeNH4(NH4)OH per liter.
Two supporting studies are available:
The toxicity of of FeNaEDTA in fish was investigated in a 96h acute test on the rainbow trout Onchorhynchus mykiss according to the OECD Guideline 203, EC method C.1 (92/69/EEC) and US EPA OPPTS 850.1075. The test item was evaluated in a static test under GLP (Bormann 2007, KEY study, Reliability 1). No mortalities of fish occurred up to a concentration of 100 mg/L within a period of 96 h. The mean measured concentration of the test item was between 80 and 120% of the nominal through the entire test period and the toxicological endpoints were evaluated using nominal concentration of the test item. The LC50 (96h) was determined to be > 100 mg/L with a probability of 99.9%. The NOEC (96 h) was determined to be 100 mg/L. Converted to the submission substance, the 96h-LC50 was > 94 mg/L.
The acute toxicity (96h-LC50) of sodium ferric EDTA towards Pimephales promelas was investigated according to EPA-600/3 -75 -009 and ASTM method E729 -80 in a multispecies test (published paper by Ewel et al, 1986, Reliability 2). The toxicity value was found to be >100 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
