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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26-10-1994 to 28-10-1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: A non GLP study according to acceptable guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Norme française AFNOR T90 301 de janvier 1983 intitulée: "ESSAIS DES EAUX"
Principles of method if other than guideline:
Determination of inhibition of the mobility of Daphnia magna following the standard method described in AFNOR T 90 301.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Thiophene-2-ethylamine
EC Number:
250-196-9
EC Name:
Thiophene-2-ethylamine
Cas Number:
30433-91-1
Molecular formula:
C6H9NS
IUPAC Name:
2-(2-thienyl)ethanamine
Constituent 2
Reference substance name:
2-Thiopheneethanamine
IUPAC Name:
2-Thiopheneethanamine
Details on test material:
- Name of test material (as cited in study report): Intermediare clopidogral (S3285)
- Lot/batch No.: 4SNS002

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
yes
Details on test solutions:
Preparation of standard solution: 250 mg of product in 250 mL of test medium

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Straus, 1820 (Clasdocera crustacea) aged less than 24h.
Origine: INERIS laboratory

Study design

Test type:
static
Water media type:
not specified
Total exposure duration:
48 h
Remarks on exposure duration:
in obscurity
Post exposure observation period:
Not applicable

Test conditions

Hardness:
No data
Test temperature:
20°C +/- 2°C
pH:
pH of the solution at 1 g/L = 10.02
Dissolved oxygen:
> 2 mg/L
Salinity:
No data
Nominal and measured concentrations:
6.8 - 9 - 13 - 19 - 269 - 36 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass tube
- fill volume: each glass tube contains 10 mL (2mL of solution for each daphnia)
- Aeration: none
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Feeding: none

TEST MEDIUM / WATER PARAMETERS
- Preparation of medium: The test medium is prepared from known analytical grade reagents
- pH: 8.0 +/- 0.2
- Conductivity: 700 +/- 20 µS/cm

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity: obscurity

EFFECT PARAMETERS MEASURED: effective initial concentration that that immobilizes in 48h, 50% of the population of Daphnia magna.

TEST CONCENTRATIONS
- Test concentrations: 6.8 - 9 - 13 - 19 - 269 - 36 mg/L
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
12.99 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence limit
Details on results:
EC50-48h determined by the method of calculation of PROBITS (confidence limits 95%): 12.99 mg/l (11.8-14.96 mg/l).
Results with reference substance (positive control):
EC50-24h determined for potassium dichromate by the method of calculation of PROBITS (confidence limits 95%):
1.32 mg/l (1.19-1.48 mg/l): week of 24-28 october 1994

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In an acute toxicity test with daphnia the EC50 of the test substance was found 12.99 mg/l at 48 h.

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