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EC number: 700-879-7 | CAS number: 1379822-00-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Nanomaterial specific surface area
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- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
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- Specific investigations
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- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-07-23 to 2013-07-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 31 May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13th April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Version / remarks:
- April 1996
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- For determination of the test item concentration, samples were taken from the testing concentrations and the control at the start and at the end of the test.
- Vehicle:
- no
- Details on test solutions:
- - Reconstituted water: ISO medium (according to OECD 202 guideline) was used as dilution water in the experiment.
Separate stock solutions of individual substances were first prepared in deionised water (prepared in the laboratory by MILLIPORE ELIX 3 water purification system). The ISO medium was prepared by adding 25 mL from each of four stock solutions to one litre deionised water.
Test solutions:
A stock solution of 100 mg/L (nominal) was first prepared by dissolving an amount of 0.05 g test item in 500 mL ISO medium using an overnight shaking (to obtain clear solution). The test solutions of the chosen test concentrations were prepared by appropriate diluting of this stock solution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Species: Daphnia magna (Straus)
- Age at test start: Less than 24 hours old
- Sex: Female
- Origin: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection) 2100 Gödöllő, Kotlán S. u. 3. Hungary
- Breeding conditions: Daphnia were bred in the Laboratory of TOXI-COOP ZRT. under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test. The test animals were fed with centrifuged green alga suspension.
ACCLIMATION
Test animals were bred under similar conditions as that used during the exposure period, therefore additional acclimatisation before the test was not necessary. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
- Hardness:
- 249 mg/L (as CaCO3)
- Test temperature:
- 20.4 – 20.8 °C (test vessels); 20.2 – 21.4 °C (ambient temperature within the climate chamber)
- pH:
- 7.18 – 8.11
- Dissolved oxygen:
- The dissolved oxygen concentration was in the range of 7.70 – 8.12 mg/L during the test.
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 6.3, 12.5, 25.0, 50.0 and 100.0 mg/L.
Measured concentrations (% of nominal) at the start of the test: 103, 95, 97, 94, 94
Measured concentrations (% of nominal) at the end of the test: 116, 112, 109, 108, 104
The analytically measured concentrations of the test item were within ± 20 % of the nominal during the experiment therefore the biological results were based on the nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers of approximately 40 mL test medium
- Number of animals: Twenty Daphnia per test group, divided into four groups of five animals, each group in ~40 mL test medium.
- Aeration: no
Pre-Experiments:
In order to select appropriate test concentrations for use in the definitive test, a preliminary range-finding test was conducted to determine the approximate toxicity of the test item.
A stock solution of 100 mg/L (nominal concentration) was first prepared by dissolving the test item in ISO medium using an overnight shaking (to obtain clear solution). The test solutions were prepared by appropriate dilution of this stock solution. Ten daphnids (divided into two replicates) in each test concentration and control were exposed for 48 hours.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: The light-dark cycle during the test was 16 hours light (artificial illumination) and 8 hours darkness.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: test concentrations: 0; 0.1; 1; 10; 100 mg/L
- Results used to determine the conditions for the definitive study: immobilized animals: 1; 0; 0; 2; 10, respectively - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 45.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Biological results:
The test item concentrations remained within ± 20 % of the nominal during the test period of 48 hours, therefore all biological results are based on the nominal concentrations.
- Results after 24 hours:
The immobilization was 100 per cent at the highest tested concentration (100 mg/L) after 24 hours of exposure. Not significant immobility was observed (up to 10 per cent) at the concentration range of 6.3 – 50.0 mg/L. In the control no immobility was noticed.
The 24-h EC50 was calculated to be 60.8 mg/L using Probit analysis by SPSS PC+ software.
- Results after 48 hours:
Not significant immobility was observed (up to 10 per cent) at the concentrations of 6.3 and 12.5 mg/L. Accordingly, the 48-h NOEC was determined to be 12.5 mg/L. In the three highest test concentrations of 25.0, 50.0 and 100 mg/L significant immobility was noticed (20, 30, and 100% respectively). Accordingly, the 48-h LOEC was determined to be 25.0 mg/L and the 48-h EC100 was determined to be 100 mg/L.
The 48-h EC50 was calculated to be 45.1 mg/L using Probit analysis by SPSS PC+ software. - Results with reference substance (positive control):
- The date of the last study with reference item Potassium dichromate was: 05 – 06 March 2013. The 24h EC50: 0.97 mg/L, (95 % confidence limits: 0.81 – 1.14 mg/L) .
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a 48-hour acute toxicity test with Daphnia magna the 48 h EC50 value was determined to be 45.1 mg/L.
- Executive summary:
The 48-hour acute toxicity test with Daphnia magna was conducted in accordance with EU method C.2, OECD guideline no. 202 and EPA OPPTS 850.1010 guideline. The 48 h-EC50 value was determined as 45.1 mg/L, and the 48 h NOEC was determined to be 12.5 mg/L. The measured concentrations of the test item varied between 94 and 116 % of the nominal concentration during the experiment.Therefore, the results are based on the nominal test item concentrations.
Reference
The measured concentrations of the test item varied between 94 and 116 % of the nominal concentration during the experiment.
Description of key information
In a 48-hour acute toxicity test with Daphnia magna the 48 h EC50 value was determined to be 45.1 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 45.1 mg/L
Additional information
The 48-hour acute toxicity test with Daphnia magna was conducted in accordance with EU method C.2, OECD guideline no. 202 and EPA OPPTS 850.1010 guideline. The 48 h-EC50 value was determined as 45.1 mg/L, and the 48 h NOEC was determined to be 12.5 mg/L. The measured concentrations of the test item varied between 94 and 116 % of the nominal concentration during the experiment.Therefore, the results are based on the nominal test item concentrations.
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