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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The 96-Hour LL*50based on nominal loading rates was 26 mg/L loading rate WAF with 95% confidence limits of 22 - 30 mg/L loading rate WAF. The Lowest Observed Effect Loading rate was 32 mg/L loading rate WAF. The No Observed Effect Loading rate was 18 mg/L loading rate WAF.

Key value for chemical safety assessment

LC50 for freshwater fish:
32 mg/L

Additional information

The key study was performed in accordance with OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008.

Following a preliminary range-finding test, fish (Rainbow trout) were exposed, in groups of seven, to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 10, 18, 32, 56 and 100 mg/L for a period of 96 hours at a temperature of approximately 14°C under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.

The 96-Hour LL*50based on nominal loading rates was 26 mg/L loading rate WAF with 95% confidence limits of 22 - 30 mg/L loading rate WAF. The Lowest Observed Effect Loading rate was 32 mg/L loading rate WAF. The No Observed Effect Loading rate was 18 mg/L loading rate WAF.

Total Organic Carbon (TOC) analysis of the test preparations at 0 (fresh media), 24 and 96 hours (old media) showed measured concentrations below the limit of quantification (assessed as 1.0 mg Carbon/L).