N-tert-butylacrylamide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of test chemical

Justification for type of information:

Experimental data of test chemical is from data from handbook

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE report is based on two short term toxicity study on microorganism for the test chemical :

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other: 1: Pseudomonas putida, 2:Mycobacterium smegmatis

Test type:

not specified

Water media type:

not specified

Total exposure duration:

18 h

Reference substance (positive control):

no

Key result

Duration:

18 h

Dose descriptor:

EC10

Effect conc.:

2 050 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 1st study

Dose descriptor:

EC0

Effect conc.:

9 707 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 2nd study

Validity criteria fulfilled:

no

Conclusions:

1st study: Based on the growth rate inhibition of Pseudomonas putida by the test chemical exposure for 18 hrs, the EC10 was determine at 2050 mg/l.
2nd study: Based on the effect observed on Mycobacterium smegmatis by the test chemical, the EC0 was determine at 9707 mg/l.
Thus based on the above effects, chemical toxicity ranges from 2050 mg/l to 9707 mg/l.

Executive summary:

Based on the experimental studies for the test chemical, studies have been were reviewed and mention as below:

 

Objective of this study was to determine the effect of test chemical on the growth of microorganism. Test conducted for 18 hrs. Based on the growth rate inhibition of Pseudomonas putida by the test chemical exposure for 18 hrs, the EC10 was determine at 2050 mg/l.

Above first study was supported by the second study from handbook. Aim of this study was to determine the effect of test chemical on the growth of microorganism Mycobacterium smegmatis. Based on the effect observed on Mycobacterium smegmatis by the test chemical, the EC0 was determine at 9707 mg/l.

Thus based on the above effects, chemical toxicity ranges from 2050 mg/l to 9707 mg/l.

Description of key information

1st study: Based on the growth rate inhibition of Pseudomonas putida by the test chemical exposure for 18 hrs, the EC10 was determine at 2050 mg/l.

2nd study: Based on the effect observed on Mycobacterium smegmatis by the test chemical, the EC0 was determine at 9707 mg/l.

Thus based on the above effects, chemical toxicity ranges from 2050 mg/l to 9707 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

2 050 mg/L

Additional information

Based on the experimental studies for the test chemical and structurally and functionally similar read across chemicals, studies have been were reviewed and mention as below:

 

Objective of this study was to determine the effect of test chemical on the growth of microorganism. Test conducted for 18 hrs. Based on the growth rate inhibition of Pseudomonas putida by the test chemical exposure for 18 hrs, the EC10 was determine at 2050 mg/l.

Above first study was supported by the second study from handbook. Aim of this study was to determine the effect of test chemical on the growth of microorganism Mycobacterium smegmatis. Based on the effect observed on Mycobacterium smegmatis by the test chemical, the EC0 was determine at 9707 mg/l.

Thus based on the above effects, chemical toxicity ranges from 2050 mg/l to 9707 mg/l.