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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH

see justification attached in section 13
Reason / purpose for cross-reference:
read-across source
Test performance:
No oxygen consumption by nitrification has been observed in the flask with test item at the end of the test.
Key result
Parameter:
% degradation (O2 consumption)
Value:
5
Sampling time:
28 d
Details on results:
All validity criteria of the test method were met:
The reference compound has reached the level for ready biodegradabiiity by 14 days.

No toxicity of the test item has been observed in the toxicity control.

The difference of extremes of replicate values of the removal of test chemical at the end of the test is less than 20 %.

The oxygen uptake of die inoculum blank is < 60 mg/1.

The pH between 6.0 and 8.5 in the test vessels at the end of the test (if degradation is less than 60 %).


Oxygen uptake by nitrification has been determined. The oxygen consumed by nitrification was 0 mg/1.
Results with reference substance:
The reference compound showed 90 % degradation after 14 days.

0% degradation after 4 days.

1% degradation after 6 days.

1% degradation after 8 days.

1% degradation after 12 days.

3% degradation after 14 days.

4% degradation after 18days.

3% degradation after 20 days.

2% degradation after 22 days.

3% degradation after 26 days.

5% degradation after 28 days

 

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Within 28 days, a degradation of 5 % was determined.

The test substance, by analogy of read across, has to be classified as "Not Readily Biodegradable".
Executive summary:

A study was performed to assess the ready biodegradability of the strucural analogue. The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-D "Manometric Respirometry Test". This test method is in all essential parts identical with OECD Guideline 301 F.Study performed in accordance with the Principles Good Laboratory Practice (GLP).

A solution of the test substance in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period the ready biodegradability is determined.

The structural analogue showed no relevant biodegradation throughout the testing period; therefore, the test substance, by analogy of read across,has to be classified as "Not Readily Biodegradable"

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 October 2003 to 13 November 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Type: mixed population of aquatic microorganisms (activated sludge)
Origin: aeration tank of a waste water plant treating predominantly domestic sewage (Wupper area water authority)
Date of collection: 2003-10-15
Pre-treatment: none
Concentration of inoculums: 30mg/lss
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
ThOD/L
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Pre-treatment of the test item: 25 mg test item were weighed out on aluminium foil. This substance inclusive the aluminium foil was added to the test vessels to give a test concentration of 100 mg/1 test item.

Exposure conditions
Test volume: 250 ml
Test apparatus: Voith Sapromat
Mixing: 1 magnetic stirrer per test vessel
Incubation time: 28 days
Incubation temperature: 22 + 2°C
Reference substance:
benzoic acid, sodium salt
Preliminary study:
No data
Test performance:
No oxygen consumption by nitrification has been observed in the flask with test item at the end of the test.
Parameter:
% degradation (O2 consumption)
Value:
5
Sampling time:
28 d
Details on results:
All validity criteria of the test method were met:
The reference compound has reached the level for ready biodegradabiiity by 14 days.

No toxicity of the test item has been observed in the toxicity control.

The difference of extremes of replicate values of the removal of test chemical at the end of the test is less than 20 %.

The oxygen uptake of die inoculum blank is < 60 mg/1.

The pH between 6.0 and 8.5 in the test vessels at the end of the test (if degradation is less than 60 %).


Oxygen uptake by nitrification has been determined. The oxygen consumed by nitrification was 0 mg/1.
Results with reference substance:
The reference compound showed 90 % degradation after 14 days.

0% degradation after 4 days.

1% degradation after 6 days.

1% degradation after 8 days.

1% degradation after 12 days.

3% degradation after 14 days.

4% degradation after 18days.

3% degradation after 20 days.

2% degradation after 22 days.

3% degradation after 26 days.

5% degradation after 28 days

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Within 28 days, a degradation of 5 % was determined.

The test substance has to be classified as "Not Readily Biodegradable".
Executive summary:

A study was performed to assess the ready biodegradability of the test substance. The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-D "Manometric Respirometry Test". This test method is in all essential parts identical with OECD Guideline 301 F.Study performed in accordance with the Principles Good Laboratory Practice (GLP).

A solution of the test substance in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period the ready biodegradability is determined.

The test substance showed no relevant biodegradation throughout the testing period; therefore, the test substance has to be classified as "Not Readily Biodegradable"

Description of key information

The structural analogue showed no relevant biodegradation throughout the testing period; therefore, the test substance, by analogy of read across, has to be classified as "Not Readily Biodegradable"

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information

A study was performed to assess the ready biodegradability of the strucural analogue.The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-D "Manometric Respirometry Test". This test method is in all essential parts identical with OECD Guideline 301 F.Study performed in accordance with the Principles Good Laboratory Practice (GLP).

A solution of the test substance in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period the ready biodegradability is determined.

The structural analogue showed no relevant biodegradation throughout the testing period; therefore, the test substance, by analogy of read across,has to be classified as "Not Readily Biodegradable"