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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
09 July 2007 to 10 August 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
Sampling schedule:
Control : at 0 and 48 hours
Test concentrations : at 0 and 48 hours

Vehicle:
no
Details on test solutions:
105.1 mg of the test item were added to 1 litre of dilution water.

Culture and dilution water
Reconstituted water (so-called 'M4 medium', originally described in Water Research 24 (9), September 1990: 1157-1167), prepared according to the recommendations of Bundesgesundheitsamt Berlin. This standard dilution water is used for both, the maintenance of the test animals and the preparation of stock and test solutions of the test item. The total hardness of the dilution water, measured at test start, was 15.4°dH (= 275 mg/L CaCO3).
Test organisms (species):
Daphnia magna
Details on test organisms:
Name : Daphnia magna STRAUS, parthenogenetic females
Source : Strain of Bundesgesundheitsamt Berlin
Maintenance and Acclimatisation : A population of parthenogenetic females of synchronised age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') is partly renewed once a week. The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period are recorded daily in a semi-quantitative way. The neonates are separated from their parent Daphnia by filtration prior to the acute test.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
No post exposure observation period
Hardness:
275 mg CaCO3/L
Test temperature:
20.9C
pH:
7.7
Dissolved oxygen:
8.6 mg/l
Salinity:
Not applicable.
Nominal and measured concentrations:
0 and 100 mg/l
Details on test conditions:
Test vessels : 50 ml glass beakers holding 10 neonates in 20 ml of test medium
Experimental design : 1 test concentration plus 1 control
10 neonates per vessel, 2 replicates per concentration/control
no feeding during the exposure period
static system
Method of initiation : neonates were placed in prepared media
Photoperiod : 16 h light : 8 h dark
Temperature : mean +/- 1 °C (in the temperature range 18-22 °C)
Aeration : none
Test item concentration/s : 100 mg/l
Method of administration : direct weighing
Medium renewal : none
Duration of exposure : 48 hours
Criteria of effects : The criterion of adverse effects used in this study was the item-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No toxic effects against daphnia at a test concentration of 100 mg/l.
The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively.
Results with reference substance (positive control):
Not applicable.
Reported statistics and error estimates:
The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively.

RESULTS

Immobilisation forDaphnia magna

(initial population: 20)

24h

48h

Test item concentration [mg/l]: Control

Absolute

0

0

Cumulative

0

0

Cumulative [%]

0

0

Test item concentration [mg/l]: 100

Absolute

0

0

Cumulative

0

0

Cumulative [%]

0

0

 

 

Abnormalities

24h

48h

Test item concentration [mg/l]: Control

Abnormal colouration

0/20

0/20

Abnormal swimming behaviour

0/20

0/20

Test item concentration[mg/l] 100

Abnormal colouration

0/20

0/20

Abnormal swimming behaviour

0/20

0/20

Daphnids with effects / daphnids mobile             += all daphnids immobile

Validity criteria fulfilled:
yes
Conclusions:
Analysis of the immobilisation rates gives the following results:

Time EC 50
[h] [mg/l]
24 > 100
48 > 100

No toxic effects against daphnia at a test concentration of 100 mg/l.

The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively.
Executive summary:

A study was performed to assess the acute toxicity of Gelb-sulfato to Daphnia magna STRAUS under static conditions.

 

The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' which is in most parts equivalent to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC Part C, Method 2 'Acute toxicity for Daphnia'. Study performed in accordance with the Principles of Good Laboratory Practice (GLP) and reported with a GLP certificate.

 

The Daphnia were exposed to a limit test concentration of nominally 100 mg/l of Gelb-sulfato dissolved in water.

 

Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined:

 

Time EC 50

[h] [mg/l]

24 > 100

48 > 100

 

No toxic effects against daphnia at a test concentration of 100 mg/l.

 

The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively.

Description of key information

There was no toxic effects against daphnia at a test concentration of 100 mg/L. The EC0 and EC50 are above 100 mg/L.

Key value for chemical safety assessment

Additional information

A study was performed to assess the acute toxicity of Gelb-sulfato to Daphnia magna STRAUS under static conditions. The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' which is in most parts equivalent to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC Part C, Method 2 'Acute toxicity for Daphnia'. Study performed in accordance with the Principles of Good Laboratory Practice (GLP) and reported with a GLP certificate.

The Daphnia were exposed to a limit test concentration of nominally 100 mg/L of the test substance dissolved in water.

 

Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined:

 

Time       EC50

[h]          [mg/l]

24       > 100

48       > 100

The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively. 

No toxic effects against daphnia at a test concentration of 100 mg/L The EC50 (48 h): > 100 mg/L test mat. based on: mobility