Alkenes, C6-8-branched, C7-rich

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study, available as unpublished report, limitations in reporting but otherwise adequate for assessment.

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in Section 13.

Cross-reference

Reason / purpose for cross-reference:

read-across: supporting information

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study, available as unpublished report, limitations in reporting but otherwise adequate for assessment.

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in Section 13.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes

Remarks:

No certificate

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

28 d

Initial conc.:

37.54 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance:

benzoic acid, sodium salt

Parameter:

other: ThOD

Value:

> 74.1 - < 80

Sampling time:

28 d

Details on results:

10% biodegradation reached on day 3, 60.9 and 62.9% degradation on day 11.

Results with reference substance:

Reference substance showed 84.8% biodegradation after 28 days.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

n-olefins C11-12 were readily biodegradable in an OECD 301F

Executive summary:

This GLP study was conducted to standard guidelines. The analytical method used to determine oxygen consumption is not stated in the report. Despite this limitation the validity criteria of the test are met and so this study is considered suitable for use. n-olefins C11 -C12 are considered readily biodegradable as a mean of 77.1% degradation occurred within 28 days.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes

Remarks:

No certificate

Test material

Test material information

Constituent 1

Reference substance name:

C11-C12 hydrocarbons

IUPAC Name:

C11-C12 hydrocarbons

Details on test material:

mix of n-undecene and n-dodecene, colourless liquid

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not reported

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

obtained from the aeration basin of the municipal water treatment plant, Novara, Italy. Sludge aerated at room temperature for 24 hours prior to the test.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

37.54 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Inoculum concentration of 30mg/l suspended solids. Test conducted at 22±0.2°C. As the test substance is insoluble in water itwas added directly to the test flasks by a microsyringe. Duplicate test and blank controls were run and the oxygen consumption measured daily.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Preliminary study:

data not reported

Test performance:

data not reported

% Degradation

Parameter:

other: ThOD

Value:

> 74.1 - < 80

Sampling time:

28 d

Details on results:

10% biodegradation reached on day 3, 60.9 and 62.9% degradation on day 11.

BOD5 / COD results

Results with reference substance:

Reference substance showed 84.8% biodegradation after 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

n-olefins C11-12 were readily biodegradable in an OECD 301F

Executive summary:

This GLP study was conducted to standard guidelines. The analytical method used to determine oxygen consumption is not stated in the report. Despite this limitation the validity criteria of the test are met and so this study is considered suitable for use. n-olefins C11 -C12 are considered readily biodegradable as a mean of 77.1% degradation occured within 28 days.