3-({[(4-methylphenyl)sulfonyl]carbamoyl}amino)phenyl 4-methylbenzenesulfonate;N-(p-toluenesulfonyl)-N'-(3-(p-toluenesulfonyloxy)phenyl)urea

Repeated dose toxicity: oral

Administrative data

Endpoint:

sub-chronic toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 408 (Repeated Dose 90-Day Oral Toxicity Study in Rodents)

Version / remarks:

(1998)

Deviations:

no

GLP compliance:

yes

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

432-520-2

EC Name:

-

Cas Number:

232938-43-1

Molecular formula:

C21 H20 N2 O6 S2

IUPAC Name:

3-{[(4-methylbenzenesulfonyl)carbamoyl]amino}phenyl 4-methylbenzene-1-sulfonate

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Strain as stated in the report: HanIbm:WIST (SPF)
- Source: RCC Ltd. Biotechnology & Animal Breeding Division, CH-4414 Fullinsdorf /Switzerland
- Age at delivery: 6 weeks
- Age at study initiation: 7 weeks
- Weight at study initiation: 130-162g (males), 111-134g (females)
- Housing: in groups of 5 in Makrolon type-4 cages
- Diet ad libitum: pelleted standard Provimi Kliba 3433 rat maintenance diet
- Water ad libitum: tap water
- Acclimation period: 7 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22+/-3
- Humidity (%): 30-70%
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Details on oral exposure:

PREPARATION OF DOSING SOLUTIONS:
Formulations of test article in polyethylene glycol were prepared daily by homogenisation with a magnetic stirrer and were used immediately at room temperature.

VEHICLE:
- Concentration in vehicle: 0, 2.5, 5, 10 and 30 mg/ml

Analytical verification of doses or concentrations:

yes

Details on analytical verification of doses or concentrations:

Concentration, homogeneity and stability (after 3 hours) of the dose formulations were determined in samples taken after experimental start. Concentration and homogeneity of the dose formulations were further determined in samples taken at monthly intervals. The individual concentrations varied in the range from -9% to +5% of the mean concentrations. In addition, nominal concentrations were achieved in the samples and the substance was stable for at least 3 h. The analyses were performed by HPLC.

Duration of treatment / exposure:

91/92 days

Frequency of treatment:

daily

Doses / concentrations

Remarks:

Doses / Concentrations:
12.5, 25, 50 and 150 mg/kg bw/d
Basis:
actual ingested

No. of animals per sex per dose:

Groups 1 and 5 (0 and 150 mg/kg bw/d): 15 males; 15 females (10 animals of each sex were sacrificed after the last treatment. Remaining animals were sacrificed after 4 week recovery period). Groups 2, 3 and 4 (12.5, 25 and 50 mg/kg bw/d): 10 males; 10 females

Control animals:

yes, concurrent no treatment

Details on study design:

The dose selection was based on a 28 day subacute oral toxicity study with 30, 150 and 750 mg/kg bw/d (RCC 743512).
The test article was administered daily by oral gavage to rats of both sexes at dose levels of 12.5, 25, 50 and 150 mg/kg bw/d for a period of 91/92 days. A control group was treated with the vehicle only. The groups comprised 10 animals per sex which were sacrificed after 90 days of treatment. Additional 5 rats per sex and group were used at 0 and 150 mg/kg body weight/day. These animals were treated for 91 days and then allowed a 28-day treatment-free recovery period after which they were sacrificed.

Examinations

Observations and examinations performed and frequency:

CLINICAL SIGNS AND MORTALITY
The animals were observed for clinical signs once daily. Detailed clinical observations were performed once before study begin and weekly thereafter. Mortality of animals was checked twice daily.
BODY WEIGHT
The weight of each animal was recorded on the day of commencement of treatment, weekly during treatment and recovery and before necropsy.
FOOD CONSUMPTION
The food consumption was recorded once during the pretest period and weekly thereafter.
OPHTHALMOSCOPIC EXAMINATION
Ophthalmoscopic examinations were performed during acclimatization on all animals, and during week 13 on animals of the control and high dose groups and during week 17 on the remaining animals of the control and high dose groups.
The ophthalmoscopic examinations of both eyes of all animals were performed after the application of a mydriatic solution using an ophthalmoscope. A description of any abnormality was recorded. For unilateral findings the contralateral eye was without abnormalities.
HEMATOLOGY AND CLINICAL CHEMISTRY
After 13 weeks (end of treatment) and 17 weeks (end of recovery) animals were fasted in metabolism cages for 18 h before blood sampling. Blood was collected under light isoflurane anesthesia from the orbital sinus for hematology and clinical chemistry. Following parameters were examined at hematology: Erythrocyte count, Reticulocyte maturity index, Hemoglobin, Mean corpuscular hemoglobin, Mean corpuscular hemoglobin concentration, Hemoglobin concentration distribution width, Hematocrit, Met-hemoglobin, Mean corpuscular volume, Total leukocyte count, Red cell volume distribution width, Platelet count, Reticulocyte count, Differential leukocyte count (neutrophils, eosinophils, basophils, lymphcytes, monocytes, large unstained cells), Coagulation (thromboplastin time, activated partial thromboplastin time).
Following parameters were examined at clinical chemistry:
Glucose, Urea, Creatinine, total Bilirubin, total Cholesterol, Triglycerides, Phospholipids, Aspartate aminotransferase, Alanine aminotransferase, Lactate dehydrogenase, Creatine kinase, Alkaline phosphatase, Gamma-glutamyl-transferase, Sodium, Potassium, Chloride, Calcium, Phosphorus (inorganic), total Protein, Protein, electrophoresis (pre-albumin, albumin, alpha-1 globulin, alpha-2 globulin, beta globulins, gamma globulins) Albumin/Globulin ratio.
URINALYSIS
After 13 weeks (end of treatment) and 17 weeks (and of recovery) urine was collected for urinalysis during the 18 h fasting period (see hematology and clinical chemistry). Following parameters were examined at urinalysis: Volume, Specific gravity, Osmolality, Color, Appearance, pH, Proteins, Glucose, Ketones, Urobilinogen, Bilirubin, Blood, Nitrites, Sediment.
FUNCTIONAL OBSERVATIONAL BATTERY
During week 13, forelimb and hind limb grip strength measurements were performed using a push-pull strain gauge. Locomotor activity was measured quantitatively for a 60-minute period and the total activity of this time period was recorded. Low beams count was reported in 15-minute intervals as well as the total activity of the measuring period.

Sacrifice and pathology:

GROSS PATHOLOGY
All surviving animals were weighed and necropsied. Macroscopic examinations were performed and samples of tissues and organs were collected and kept in fixative. Following tissues were preserved at necropsy:
Adrenals, Aorta, Bone (sternum), Bone marrow (femur), Brain (telencephalon, cerebellum, pons), Caecum, Colon, Duodenum, Esophagus, Eyes with optic nerve, Harderian gland, Heart, Ileum, Jejunum, Kidneys, Larynx, Lacrimal gland, Liver, Lungs, Lymph nodes (mesenteric, thoracic), Mammary gland, Nasal cavity, Ovaries, Pancreas, Pituitary, Prostate gland, Rectum, Salivary glands (parotid and mandibular), Sciatic nerve, Seminal vesicles, Skeletal muscle, Skin, Spinal cord, Spleen, Stomach, Testes with epididymides, Thymus, Thyroids / Parathyroids, Tongue, Trachea, Urinary bladder, Uterus (corpus, cervix), Vagina, All gross lesions.
ORGAN WEIGHTS
Following organs from all animals sacrificed at termination were weighed: Adrenals, brain, epididymides, heart kidneys, liver, ovaries, spleen, testes, thymus, thyroids/parathyroids and uterus.
HISTOPATHOLOGY
Histopathological examinations were performed in all animals at 0 and 150 mg/kg bw/day as well as in animals found dead or sacrificed moribund. Following tissues were examined histopathologically:
Adrenals, Aorta, Bone marrow (femur), Brain (telencephalon, cerebellum, pons), Caecum, Colon, Duodenum, Esophagus, Eyes with optic nerve, Heart, Ileum, Jejunum, Kidneys, Liver, Lungs, Lymph nodes (mesenteric, thoracic), Ovaries, Pancreas, Pituitary, Prostate gland, Rectum, Sciatic nerve, Seminal vesicles, Skeletal muscle, Skin, Spinal cord, Spleen, Stomach, Testes with epididymides, Thymus, Thyroids / Parathyroids, Trachea, Urinary bladder, Uterus (corpus, cervix), Vagina, All gross lesions.
Samples were embedded, cut at an approximate thickness of 2 to 4 micrometers and stained with hematoxylin and eosin. All tissues not required for microscopic examinations were kept in fixative. From the animals of the low and middle dose groups, adrenal glands, liver and spleen of both sexes were examined.

Statistics:

The following statistical methods were used to analyze the grip strength, locomotor activity, food consumption, body weight, organ weights and ratios:
- The Dunnett-test (many to one t-test) based on a pooled variance estimate was applied if the variables were assumed to follow a normal distribution for the comparison of the treated groups and the control groups for each sex.
- The Steel-test (many-one rank test) was applied instead of the Dunnett-test when the data could not be assumed to follow a normal distribution.
- Fisher's exact-test was applied to the macroscopic findings and ophthalmoscopy.
For clinical laboratory data, quantitative data was analyzed by a one-way analysis of variance (ANOVA) when the variances were considered homogeneous according to Bartlett. Alternatively, if the variances were considered to be heterogenous (p<0.05), a nonparametric Kruskal-Wallis test was used. Treated groups were then compared to the control groups using Dunnett's test if the ANOVA was significant at the 5% level and by Dunn's test in the case of a significant Kruskal-Wallis test (p<0.05).

Results and discussion

Results of examinations

Clinical signs:

no effects observed

Mortality:

no mortality observed

Body weight and weight changes:

no effects observed

Food consumption and compound intake (if feeding study):

no effects observed

Ophthalmological findings:

no effects observed

Haematological findings:

effects observed, treatment-related

Description (incidence and severity):

see below

Clinical biochemistry findings:

effects observed, treatment-related

Description (incidence and severity):

see below

Urinalysis findings:

effects observed, treatment-related

Description (incidence and severity):

see below

Behaviour (functional findings):

no effects observed

Organ weight findings including organ / body weight ratios:

effects observed, treatment-related

Description (incidence and severity):

see below

Gross pathological findings:

no effects observed

Histopathological findings: non-neoplastic:

effects observed, treatment-related

Description (incidence and severity):

see below

Details on results:

HEMATOLOGY AND CLINICAL CHEMISTRY
Hematology
After 13 week treatment with 150 mg/kg bw/d test substance, significantly lower red blood cell count, lower hemoglobin levels, reduced hematocrit and increased red cell distribution width were noted in males and females. Although most values remained within the historical control, these differences were considered to be treatment related and indicative of slight anemia.
In addition, significant increases in the absolute and relative reticulocyte counts, as well as concomitant fluorescence shift towards high fluorescence in the reticulocyte fluorescence ratios were noted in both sexes at 150 mg/kg bw/day. These differences were considered to be compensatory changes to the anemia.
The number of large unstained cells was significantly higher in females at 150 mg/kg bw/day and also exceeded the historical control data. A treatment relationship could not be excluded. However, the toxicological significance is unclear. At 12.5, 25 and 50 mg/kg bw/day the number of large unstained cells was not increased.
No further treatment related findings were noted in the hematology parameters after 13 weeks of treatment. Parameters reaching significance in either sex and/or either dose are given in table 3.
At 150 mg/kg bw/d the white blood cell count of females was significantly increased compared to control. After recovery period the white blood cell count of females was decreased (not significantly). In addition, this finding was not seen in males and was therefore considered to be incidental.
Further differences, which were considered to be incidental, consisted of: reduced relative basophil count in males treated with 50 mg/kg/day or 150 mg/kg bw/day and increased lymphocyte count in females treated with 150 mg/kg bw/day. Both findings were considered to be due to a deviant control value and therefore not treatment related. The platelet count was significantly increased in females at 150 mg/kg bw/day, but remained within the limits of the historical control data and did not show a clear dose-response relationship.
In females treated with 150 mg/kg/day, the prothrombin and partial thromboplastin times were significantly increased when compared with control. The increased prothrombin time was also noted in females treated with 12.5 mg/kg bw/day.
After recovery (4 weeks), the elevated hematocrit persisted in males of the high dose group. All other findings were considered to be reversible.
Further findings after recovery consisted of lower mean corpuscular hemoglobin concentrations, increased platelet count and number of large unstained cells in males as well as decreased met-hemoglobin in females. These parameters were unaffected after treatment and are considered to be incidental.
Clinical Chemistry:
Treatment related changes in the clinical chemistry were seen at 150 mg/kg bw/day only.
At 150 mg/kg bw/d inorganic phosphorus levels were significantly increased in males after treatment and after recovery (2.16 vs. 1.99 mmol/l at control and 1.99 vs. 1.78 mmol/l at control). Although this effect was not seen in females, a treatment relationship could not be excluded. In addition, at 150 mg/kg bw/d significantly increased total bilirubin levels were seen in females (3.12 vs. 1.85 µmol/l at control).
Further treatment related differences were seen in the absolute protein fractions or in the relative fractions of protein electrophoresis. The findings consisted of:
-increased relative alpha globulins in females (0.181 vs. 0.171 at control for alpha1 globulin and 0.701 vs. 0.062 at control for alpha2 globulin).
-increased absolute alpha globulins in females (13.05 vs. 12.03 g/l at control for alpha1 globulin and 5.09 vs. 4.35 g/l at control for alpha2 globulin, respectively)
-increased relative beta globulin in males treated with 150mg/kg bw/day after treatment and after recovery (0.193 vs. 0.179 at control and 0.183 vs. 0.165 at control, respectively).
-increased absolute beta globulin level in males after treatment and recovery (13.18 vs. 12.05 g/l at control and 12.22 vs. 10.74 g/l at control, respectively).
-the relative albumin levels were reduced in females treated with 150 mg/kg bw/day, and subsequently the albumin/globulin ratio was slightly reduced in females at 150 mg/kg bw/day (1.295 vs. 1.427).
In addition, in females significantly increased alpha2 globulins were noted at 12.5 and 50 mg/kg bw/d after treatment. Since these effects were not dose dependent they were considered to be incidental.
Further significant effects, such as increased creatinine level, cholesterol levels, alanine aminotransferase, alkaline phosphatase activity and decreased chloride levels were seen. These effects were however considered to be of no toxicological relevance, since they were either within the historical control data, not dose dependent, resulted from an unusually high or low control value or were only seen after recovery but not after treatment.
All other clinical biochemistry parameters were considered to be unaffected.

Significant findings seen after the recovery period were considered to be incidental rather than treatment related. Effects were either within the range of the historical control data or were caused by a high control value. Findings consisted of:
-elevated sodium level in males at 150 mg/kg bw/day
-decreased potassium level in males at 150 mg/kg bw/day
-marginally reduced urea in males at 150 mg/kg bw/day. This decrease was not within the range of the historical control. However, no differences were noted after 13 weeks and the possibility of a late treatment related effect was considered unlikely.
URINALYSIS
The urine output of males was increased at 150 mg/kg bw /day 9.57 vs. 7.69 ml after 13 weeks. The difference exceeded the limits of the historical control data. Thus, a treatment relationship could not be excluded. The increased urine output was fully reversible after recovery.
Other differences such as increased urinary pH values in males at 50 and 150 mg/kg bw/d), reduced erythrocyte count in females at 150 mg/kg bw/d and increased leukocyte count in males at 150 mg/kg bw/d, were considered to be incidental. Differences were either within the range of the historical control data, showed no clear dose response relationship or were only seen after recovery but not after treatment.
All other urinalysis parameters compared favorably with the controls.
ORGAN WEIGHTS
After 13 weeks increased relative and absolute liver weights (both sexes) and increased kidney weights (females) were seen at 150 mg/kg bw/d. The liver-to-brain weight ratios were also increased in these rats but these changes did not attain statistical significance. Details are given in table 4.
The absolute and relative liver weights of females dosed with 12.5 and 50 mg/kg bw/day were significantly increased. Since absolute and relative organ weights of females at 25 mg/kg bw/day were only marginally higher than those of the controls and the liver weights of females treated with 12.5 or 50 mg/kg/day were comparable (i.e. no clear dose-response relationship), these differences were considered to be not treatment related.
A significantly increased relative kidney weight was noted in females treated at 150
mg/kg bw/day in comparison to control. In the absence of morphologic findings, this finding was considered to be incidental. The kidney weights of the females treated with 12.5, 25 and 50 mg/kg/day were unaffected.
After 17 weeks the increased absolute and relative liver weights persisted in females at 150 mg/kg bw/d in comparison to control. The liver-to-brain weight ratio of these females was significantly higher than the control females. These changes were considered to be indicative of partial reversibility of findings noted after 13 weeks. Significantly elevated absolute kidney weights were noted in males at150 mg/kg bw/day compared to controls. Since the relative kidney weight was comparable to control, this difference was considered to be a body weight effect. In addition the kidney-to-brain weight ratio was significantly increased in these males, when compared with the controls.
No further changes in organ weights were seen after 17 weeks.
HISTOPATHOLOGY
After 13 weeks changes considered to be treatment related were present in the adrenal glands, liver and spleen of animals of both sexes given 150 mg/kg/day and in the liver of female rats given 50 mg/kg/day.
At 150 mg/kg bw/d a greater incidence and severity of minimal or slight increased cortical coarse vacuolation of adrenal glands was seen in 9/10 males and 10/10 females.
A minimal or slight hypertrophy of centrilobular hepatocytes in 9/10 males, 10/10 females given 150 mg/kg bw/day and 3/10 females given 50 mg/kg bw/day was seen. The finding correlated with the increased liver weight seen in animals of both sexes given at 150 mg/kg bw/d and females given 50 mg/kg bw/day.
At 150 mg/kg bw/d the level of extramedullary hematopoesis of the spleen was increased compared to control in 7/10 males and 7/10 females.
No treatment related histopathological changes were seen in animals given 12.5 or 25 mg/kg bw/day.
After recovery the hypertrophy of centrilobular hepatocytes and the level of extramedullary hematopoiesis of the spleen were fully reversible. The incidence of increased cortical coarse vacuolation was, however, still increased in males (2/5and females (2/5) compared to controls (0/5 both sexes).

Effect levels

1

Dose descriptor:

NOEL

Effect level:

25 mg/kg bw/day (actual dose received)

Based on:

test mat.

Sex:

male/female

Basis for effect level:

other: No effects were seen

2

Dose descriptor:

NOAEL

Effect level:

50 mg/kg bw/day (actual dose received)

Based on:

test mat.

Sex:

female

Basis for effect level:

other: Hypertrophy of centrilobular hepatocytes was noted in females only.

3

Dose descriptor:

LOAEL

Effect level:

150 mg/kg bw/day (actual dose received)

Based on:

test mat.

Sex:

male/female

Basis for effect level:

other: see 'Remark'

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

Table 3: Changes in selected hematological parameters after 13 weeks and after recovery (17 weeks).

Parameter	MALES							FEMALES
	13 weeks					17 weeks		13 weeks					17 weeks
	Dose (mg/kg bw/d)							Dose (mg/kg bw/d)
	0	12.5	25	50	150	0	150	0	12.5	25	50	150	0	150
RBC (T/l)	8.72	8.67	8.50	8.58	8.08**	8.58	9.92	7.64	7.67	7.49	7.51	7.08**	7.71	7.83
HB (mmol/l)	9.46	9.40	9.31	9.29	8.93**	9.60	9.76	8.87	8.93	8.75	8.80	8.28**	9.30	9.54
HCT (rel)	0.47	0.47	0.46	0.47	0.45**	0.48	0.50**	0.44	0.44	0.44	0.44	0.42**	0.46	0.47
RDW (rel)	0.13	0.13	0.14*	0.13	0.14**	0.12	0.12	0.12	0.12	0.12	0.12	0.13**	0.11	0.13
MCHC (mmol/l)	19.97	20.01	20.18	19.94	19.99	19.94	19.51*	20.11	20.09	21.00	19.92	19.81	20.20	20.47
LUC (rel)	0.005	0.006	0.004	0.007	0.006	0.005	0.001	0.005	0.006	0.005	0.005	0.006	0.006	0.005
LUC (G/l)	0.027	0.031	0.024	0.036	0.037	0.020	0.032*	0.0147	0.016	0.012	0.014	0.023*	0.02	0.014
Platelets (G/l)	870	943	908	939	986	755	891*	905	1011	848	906	1039*	778	804
PT (rel)	0.880	0.870	0.895	0.850	0.892	0.01	0.009	0.953	0.986	0.967	0.950	1.018**	0.902	0.958
APTT (sec)	19.15	18.79	19.06	19.27	18.64	18.44	18.75	19.12	20.24*	19.89	19.88	20.49**	19.22	18.58
Retic (%)	0.023	0.021	0.024	0.023	0.030**	0.020	0.017	0.030	0.030	0.029	0.028	0.036**	0.018	0.017
Retic (G/l)	198	181	202	193	238**	167	155	226	226	214	208	257*	142	129
HFR (%)	0.44	0.39*	0.45	0.45	0.56**	0.37	0.34	0.49	0.46	0.48	0.51	0.60**	0.35	0.34
MFR (%)	0.237	0.262	0.247	0.241	0.199**	0.259	0.281	0.225	0.238	0.230	0.216	0.195	0.266	0.269
LFR (%)	0.321	0.350	0.304	0.307	0.245**	0.369	0.376	0.283	0.301	0.291	0.277	0.206**	0.385	0.390
MET-HB (%)	0.009	0.009	0.009	0.009	0.009	0.010	0.009	0.010	0.010	0.010	0.010	0.010	0.010	0.008*
WBC (G/l)	5.25	5.44	5.06	5.08	6.13	4.53	5.77	2.95	2.84	2.68	2.93	3.70**	0.349	0.341
Eo (rel)	0.019	0.018	0.021	0.016	0.018	0.023	0.016	0.027	0.023	0.021	0.023	0.018	0.025	0.024
Eo (G/l)	0.102	0.096	0.105	0.080	0.100	0.094	0.090	0.078	0.061	0.057	0.065	0.070	0.078	0.064
Baso (rel)	0.008	0.006	0.007	0.005*	0.005**	0.008	0.007	0.005	0.005	0.005	0.005	0.003	0.009	0.008
Baso (G/l)	0.042	0.031	0.035	0.027	0.031	0.036	0.042	0.013	0.015	0.013	0.015	0.014	0.028	0.022
Lymph (rel)	0.744	0.767	0.728	0.766	0.745	0.689	0.734	0.727	0.700	0.687	0.730	0.749	0.719	0.701
Lymph (G/I)	3.935	4.198	3.680	3.900	4.633	3.172	4.260	2.17	2.03	1.84	2.13	2.91**	2.35	1.89
Mono (G/l)	0.107	0.102	0.094	0.103	0.110	0.100	0.132	0.058	0.057	0.059	0.056	0.084*	0.072	0.056
*/** Dunnet-test p<0.05 or p<0.01
Abbreviations: RBC = Erythrocyte count, HB = Hemoglobin, HCT = Hematocrit, RDW = red cell distribution width, MCHC = Mean corpuscular hemoglobin concentration, LUC = Large Unstained Cells, PLATELETS = Platelet count, RETIC = Reticulocyte count, HFR = high, MFR = middle, LFR = low Reticulocyte fluorescence ratios, MHT-HB = Methemoglobin, WBC = Total leukocyte count, PT = Thromboplastin time(=prothrombin time), APTT = Activated partial thrombopastin time, Eo = Eosinophile count, Baso = Basophile count, Lymph = Lymphocyte count

Table 4: Absolute and relative organ weights (g and g/100g bw, respectively) after treatment (week 13) and recovery (week 17).

Organ		MALES							FEMALES
		13 weeks					17 weeks		13 weeks					17 weeks
		Dose (mg/kg bw/d)							Dose (mg/kg bw/d)
		0	12.5	25	50	150	0	150	0	12.5	25	50	150	0	150
Body weight	Abs.	377	368	384	368	374	379	420	220	225	218	224	212	230	227
Thymus	Abs.	0.32	0.30	0.29	0.28	0.28	0.21	0.27	0.27	0.27	0.27	0.27	0.24	0.263	0.21
	Rel.	0.09	0.08	0.08	0.08	0.07	0.06	0.06	0.12	0.12	0.12	0.12	0.12	0.10	0.09
Thyroids	Abs.	0.02	0.02	0.03	0.02	0.02	0.03	0.03	0.02	0.02	0.02	0.02	0.02	0.02	0.02
	Rel.	0.01	0.01	0.01	0.01	0.01	0.01	0.01	0.01	0.01	0.01	0.01	0.01	0.01	0.01
Heart	Abs.	1.04	1.04	1.05	0.95	1.00	1.03	1.11	0.73	0.73	0.73	0.75	0.74	0.79	0.77
	Rel.	0.28	0.28	0.28	0.26	0.27	0.28	0.27	0.33	0.33	0.34	0.34	0.35	0.34	0.34
Brain	Abs.	2.02	2.03	2.04	2.05	2.06	2.06	2.12	1.91	1.94	1.92	1.91	1.90	1.96	1.87
	Rel.	0.54	0.56	0.54	0.56	0.55	0.55	0.51	0.87	0.87	0.88	0.86	0.90	0.86	0.83
Adrenal glands	Abs.	0.06	0.06	0.06	0.06	0.05	0.06	0.06	0.07	0.08	0.07	0.07	0.07	0.07	0.07
	Rel.	0.016	0.016	0.016	0.016	0.014	0.017	0.015	0.03	0.04	0.03	0.03	0.03	0.03	0.03
Liver	Abs.	9.26	9.33	9.78	9.37	10.43	8.65	10.04	5.54	6.43*	5.99	6.46*	7.03**	5.73	6.10
	Rel.	2.45	2.53	2.54	2.55	2.79**	2.29	2.40	2.51	2.88**	2.75	2.88**	3.31**	2.49	2.70
Spleen	Abs.	0.72	0.71	0.73	0.70	0.73	0.71	0.81	0.54	0.51	0.55	0.55	0.55	0.60	0.53
	Rel.	0.192	0.193	0.191	0.188	0.196	0.186	0.192	0.24	0.23	0.25	0.25	0.26	0.26	0.23
Kidneys	Abs.	2.24	2.28	2.27	2.18	2.18	2.13	2.42*	1.44	1.53	1.48	1.53	1.56	1.46	1.52
	Rel.	0.60	0.62	0.60	0.59	0.58	0.57	0.58	0.65	0.69	0.68	0.68	0.74**	0.63	0.67
Testes	Abs.	3.59	3.71	3.83	3.66	3.73	3.81	3.66
	Rel.	0.96	1.01	1.00	1.00	1.00	1.01	0.88
Epididymides	Abs.	1.45	1.45	1.48	1.40	1.42	1.51	1.48
	Rel.	0.385	0.394	0.389	0.381	0.383	0.403	0.357
Ovaries	Abs.								0.102	0.118	0.106	0.113	0.096	0.098	0.124
	Rel.								0.05	0.05	0.05	0.05	0.05	0.04	0.06
Uterus	Abs.								0.99	0.98	1.12	1.01	1.20	0.87	0.89
	Rel.								0.45	0.44	0.51	0.45	0.57	0.38	0.39
*/** Dunnett-test *, p<0.05; **,p<0.01;

Applicant's summary and conclusion

Conclusions:

Based on the results of this study, 25 mg/kg body weight/day was established as the NOEL and 50 mg/kg body weight/day as the NOAEL.