Tetrachloro-1,2-difluoroethane

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

July-August 1966

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Details on test material and procedure are given, the method is scientifically valid.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1966

Materials and methods

Test guideline

Guideline:

other: not reported

Principles of method if other than guideline:

not reported

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Tetrachloro-1,2-difluoroethane

EC Number:

200-935-6

EC Name:

Tetrachloro-1,2-difluoroethane

Cas Number:

76-12-0

Molecular formula:

C2Cl4F2

IUPAC Name:

1,1,2,2-tetrachloro-1,2-difluoroethane

Test material form:

other: solid

Details on test material:

- Name of test material (as cited in study report): 1,1,2,2-Tetrachloro-1,2-Difluoroethane, TCDF
- Substance type: pure substance
- Physical state: solid
- Analytical purity: 96.6 %
- Impurities (identity and concentrations): 1,1,1,2-Tetrachloro-2,2-Difluoroethane = 1.9%, unspecified fluoroalkanes = 1.5 %
- Isomers composition: 1,1,1,2-Tetrachloro-2,2-Difluoroethane = 1.9%
- Lot/batch No.: Sample No. 2905
- Expiration date of the lot/batch: not reported
- Stability under test conditions: stable
- Storage condition of test material: not reported

Test animals

Species:

rat

Strain:

other: ChR-CD

Sex:

male

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: undiluted or as peanut oil solution

Doses:

Doses ranged from 670 mg/kg to 25000 mg/kg.

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LDLo

Effect level:

> 25 000 mg/kg bw

Based on:

test mat.

Any other information on results incl. tables

The oral ALD for 1,1,2,2,-tetrachloro-1,2 -difluoroethane was > 25000 mg/kg bw.

Rats receiving doses of 11000 mg/kg bw and above showed initial weight losses and diarrhea the day after dosing.

After doses below 11000 mg/kg bw, the weight loss was slight, diarrhea was observed the day after the dosing, and some rats appeared to be hyperactive.

There were no clinical effect at 670 mg/kg bw.

There were no pathologic changes, 14 days after dosing, in the brain, spinal cord, heart, liver, kidneys, adrenals, testes, stomach, duodenum, bone marrow, spleen and thymus of rats given 11000 mg/kg bw, 17000 mg/kg bw or 25000 mg/kg bw attributable to 1,1,2,2 -tetrachloro-1,2 -difluoroethane.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

According to the reported results, the LoLD of 1,1,2,2,-tetrachloro-1,2 -difluoroethane by oral route is higher than 25000 mg/kg bw.

Executive summary:

The objective of the paper was to compare the toxicological properties of the isomers 1,1,2,2-tetrachloro-1,2-difluoroethane and 1,1,1,2-tetrachloro-2,2-difluoroethane and to this purpose a set of toxicological tests was conducted and discussed.

In order to assess the toxicity by oral route, both the isomers were administered to male rats and the approximate lethal dose ALD (i.e. the lowest dose at which mortality occurs) was determined by the method of Deichmann and LeBlanch. Pathologic examinations were conducted 14 days after the doses were given.

1,1,2,2-tetrachloro-1,2-difluoroethane was administered in the dose-range of 670 – 25000 mg/kg bw.

The oral ALD for 1,1,2,2,-tetrachloro-1,2 -difluoroethane was > 25000 mg/kg bw. Rats receiving doses of 11000 mg/kg bw and above showed initial weight losses and diarrhea the day after dosing. After doses below 11000 mg/kg bw, the weight loss was slight, diarrhea was observed the day after the dosing, and some rats appeared to be hyperactive. There were no clinical effect at 670 mg/kg bw.

There were no pathologic changes, 14 days after dosing, in the brain, spinal cord, heart, liver, kidneys, adrenals, testes, stomach, duodenum, bone marrow, spleen and thymus of rats given 11000 mg/kg bw, 17000 mg/kg bw or 25000 mg/kg bw attributable to 1,1,2,2 -tetrachloro-1,2 -difluoroethane.