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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

96 h LL50 = > 1000 mg/L; 96 h NOELR = 1000 mg/L, Oncorhynchus mykiss, OECD 203, EU Method C.1, Wetton (2002a)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1 000 mg/L

Additional information

The acute toxicity of the test material to fish was investigated in Oncorhynchus mykiss (rainbow trout) in accordance with standardised guidelines OECD 203 and EU Method C.1. Following a range finding study, 30 fish were exposed to a Water Accommodated Fraction (WAF) of the test material at a single nominal loading rate of 1000 mg/L for a period of 96 hours under semi-static conditions. The number of mortalities and sub-lethal effects of exposure in each test and control vessel were determined 3, 6, 24, 48, 72 and 96 hours after the start of the exposure. Under the conditions of the study, there were no mortalities nor sub-lethal effects at a 1000 mg/L loading rate WAF for a period of 96 hours. The LL50 was therefore determined to be in excess of 1000 mg/L loading rate WAF and the No Observed Effect Loading rate determined to be 1000 mg/L loading rate WAF. Samples of the control and 1000 mg/L loading rate WAF were taken at 0 (fresh media) and 24 hours (old media) for Total Organic Carbon (TOC) analysis. The results indicated that a soluble component of the test material was present in the test preparations. Since the test material was a mixture of components, and given that toxicity could therefore not be attributed to a single component or a mixture of components but to the test material as a whole, the results were based on nominal loading rates only.

The study was performed in line with GLP and accepted standardised guidelines with a high standard of reporting. The study was assigned a reliability score of 1 and considered suitable for assessment as an accurate reflection of the test material.

The available data are considered to be complete and the results determined, 96 h LL50 value of greater than 1000 mg/L loading rate WAF and 96 h NOELR value of 1000 mg/L loading rate WAF, were taken forward for risk assessment.