Reaction mass of 2-{[2-(2-hydroxyethoxy)ethoxy]carbonyl}benzoic acid and 2,2'-oxydiethanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-02-28 till 2012-03-26

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Concentration of 100 mg/L was measured at 0 and 48 h; and concentration of 0 mg/L at 48 h only.
- Sample storage conditions before analysis: Routinely, the samples were analysed immediately. Only in exceptional cases, they were stored overnight deep frozen and protected from light.

Vehicle:

no

Details on test solutions:

A direct weighing was prepared to produce the only test concentration. 105.4 mg of the test item were added to 1 litre of dilution water, treated for 60 seconds at 8000 rpm with an ultra turrax and stirred for 24 h on a magnetic stirrer. Finally undissolved particles of the test item were removed by filtration using a folded filter with a pore size of 7-12 μm. The pH was measured to be 7.5.
19 mL of the solution were taken and diluted with 1 mL of dilution water containing 10 daphnids resulting in a final concentration of 100 mg/L. For each test item concentration and the control 2 replicates were prepared.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Name : Daphnia magna STRAUS, parthenogenetic females
- Source : Strain of Bundesgesundheitsamt Berlin
- Maintenance and Acclimatisation : A population of parthenogenetic females of synchronized age structure has been maintained for more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (light intensity: < 20 μE x m-2 x s-1). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent Daphnia by filtration prior to the acute test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

14.7 °dH (= 262 mg/L CaCO3)

Test temperature:

21.5 - 20.5 °C measured at each test vessel at the beginning and the end of the test

pH:

7.6 - 8.0 measured at each test vessel at the beginning and the end of the test

Dissolved oxygen:

8.5 - 8.9 mg/L O2 with 92 -97 % O2 saturation measured at each test vessel at the beginning and the end of the test

Nominal and measured concentrations:

100 mg/L (nominal) plus control
The results are expressed in terms of nominal concentrations (at 24 h and at 48 h). Effective concentrations correspond to 104.2 % of nominal values at 0 hours and to 80.2 % of nominal values at 48 hours.

Details on test conditions:

PRETREATMENT OF THE TEST ITEM:
- 105.4 mg of the test item added to 1 litre of dilution water
- treated with an ultra turrax at 8000 rpm for 60 seconds
- stirred for 24 h on a magnetic stirrer
- 19 mL of the solution were taken and diluted with 1 mL of dilution water containing 10 daphnids resulting in a final concentration of 100 mg/L

TEST SYSTEM:
- Test vessels : 50 mL glass beakers covered with watch glasses holding 10 neonates in 20 mL of test medium
- 1 test concentration plus 1 control
- 10 neonates per vessel, 2 replicates per concentration/control
- no feeding during the exposure period
- Photoperiod : 16 h light : 8 h dark
- Temperature of incubation unit : 19.0 to 19.6 °C
- Aeration : none
- Medium renewal : none
- Criteria of effects : The criterion of adverse effects used in this study was the item-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inmobilisations rate

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inmobilisations rate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inmobilisations rate

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inmobilisations rate

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inmobilisations rate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inmobilisations rate

Validity criteria fulfilled:

yes

Remarks:

(- The immobilisation and other abnormalities in the controls did not exceed 10 % by the end of the test. - The dissolved oxygen concentration remained above 3 mg/L throughout the exposure period.)

Conclusions:

Daphnia magna was exposed und static conditions for 48 hours to one concentration (100 mg/L) of Ester PSA + DEG. No effect was observed (Neuhahn, 2012)

Executive summary:

In order to test acute toxicity to invertebrates (Daphnia magna) of Ester PSA + DEG was exposed to the test solution of 1 nominal concentration of the test substance (100 mg/L) and blank control solution for a period of 48 hours under static conditions. Mobility and visible abnormalities were recorded at 24 and 48 hours. The measured concentrations confirmed that deviation from the nominal concentrations was less then 21 % (measured concentrations were in the range of 80.2 - 104.2 % of nominal concentrations. No toxic effect and abnormal behavior were observed. This toxicity study is classified as appectable and satisfies the guideline requirements for acute daphnia study (Neuhahn, 2012).

Description of key information

Daphnia magna was exposed und static conditions for 48 hours to one concentration (100 mg/L) of test substance. No effect was observed (Neuhahn, 2012)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L

Additional information

In order to test acute toxicity to invertebrates (Daphnia magna) of test substance was exposed to the test solution of 1 nominal concentration of the test substance (100 mg/L) and blank control solution for a period of 48 hours under static conditions. Mobility and visible abnormalities were recorded at 24 and 48 hours. The measured concentrations confirmed that deviation from the nominal concentrations was less then 21 % (measured concentrations were in the range of 80.2 - 104.2 % of nominal concentrations. No toxic effect and abnormal behavior were observed.