1,3,5-triisopropylbenzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,3,5-triisopropylbenzene

EC Number:

211-941-3

EC Name:

1,3,5-triisopropylbenzene

Cas Number:

717-74-8

Molecular formula:

C15H24

IUPAC Name:

1,3,5-tris(propan-2-yl)benzene

Test animals

Species:

guinea pig

Strain:

Hartley

Test system

Type of coverage:

occlusive

Preparation of test site:

other: clipped

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

0.5 mL

Duration of treatment / exposure:

4 hours

Observation period:

10 days

Number of animals:

5

Details on study design:

The backs of the guinea pigs were clipped and the material was applied to the entire back area under occlusion created by application of a woven pad and a rubber dental dam over the application site. The material was left in contact with the skin for 4 hours, then washed off. Animals were assessed for skin irritation upon patch removal and for the next few days. Dermal toxicity and lethality were assessed by recording any clinical signs which subsequently evolved over a period of 10 days. As with the oral studies, weight loss was considered a sign of toxicity and an indication of absorption of the test chemical.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

other: 24 h

Score:

1

Max. score:

4

Reversibility:

fully reversible

Results 2

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

other: 24h

Score:

2

Max. score:

4

Reversibility:

fully reversible

Results 3

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

other: 24h

Score:

2

Max. score:

4

Reversibility:

fully reversible

Results 4

Irritation parameter:

erythema score

Basis:

animal #4

Time point:

other: 24 h

Score:

1

Max. score:

4

Reversibility:

fully reversible

Results 5

Irritation parameter:

erythema score

Basis:

animal #5

Time point:

other: 24h

Score:

1

Max. score:

4

Reversibility:

fully reversible

Results 6

Irritation parameter:

edema score

Basis:

animal #1

Time point:

other: 24h

Score:

1

Max. score:

4

Reversibility:

fully reversible

Results 7

Irritation parameter:

edema score

Basis:

animal #2

Time point:

other: 24h

Score:

1

Max. score:

4

Reversibility:

fully reversible

Results 8

Irritation parameter:

edema score

Basis:

animal #3

Time point:

other: 24h

Score:

1

Max. score:

4

Reversibility:

fully reversible

Results 9

Irritation parameter:

edema score

Basis:

animal #4

Time point:

other: 24h

Score:

1

Max. score:

4

Reversibility:

fully reversible

Results 10

Irritation parameter:

edema score

Basis:

animal #5

Time point:

other: 24h

Score:

1

Max. score:

4

Reversibility:

fully reversible

Applicant's summary and conclusion

Interpretation of results:

slightly irritating

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

The test material, 1,3,5-triisopropylbenzene (TIPB) is slightly irritating to guinea pig skin

Executive summary:

The potential for 1,3,5 -triisopropylbenzene (TIPB) to induce dermal irritation was evaluated in guinea pigs. The hair was clipped from the back of 5 guinea pigs, and a single 0.5 mL sample of TIPB was applied to the area under an occlusive dressing. After 4 hours, the material was washed from the skin, and the area was observed for erythema and edema for the following 2 weeks. At 24 hours after application, mild to moderate erythema and slight edema was observed in 5 of 5 guinea pigs. At the end of the treatment period, there was no sign of erythema or edema in any of the test animals. From these data, it was concluded that TIPB is slightly irritating to the skin of guinea pigs.