Reaction mass of N,N’-ethane-1,2-diylbis(12-hydroxyoctadecan-1-amide), Octadecanamide, 12-hydroxy-N-[2-[(1-oxooctadecyl)amino]ethyl]- and Octadecanoic acid, 12-hydroxy-, 1-hexyl-12-[[2-[(12-hydroxy-1-oxooctadecyl)amino]ethyl]amino]-12-oxododecyl ester

Toxicity to terrestrial plants

Description of key information

In the terrestrial plant study by Ripperger (2018) using the read-across substance CAS 100454 -48 -0, seedling emergence and seedling growth of four dicotyledonous and two monocotyledonous species were assessed. No statistically significant differences could be determined for the parameter seedling emergence and post-emergence mortality.

Statistically significant effects on shoot dry weight could be detected forLolium perenneat 1.0, 10.7 and 23.4 mg test item/kg dw. The statistically significant difference to the control at 1.0 mg test item/kg dw was considered by the laboratory as not treatment-related, since the replicate values were still within the control range and no significant effects were detected at the higher concentrations of 2.2 and 4.84 mg test item/kg dw. The highest inhibition on shoot dry weight compared to the control was determined forAllium cepawith 34.8 % at 10.7 mg test item/kg dw. Shoot dry weight is a variable parameter and some degree of non-treatment related differences may be detected across the wide range of species and replicates. The laboratory suggests a LOEC of 1 mg/kg (13% difference from the control), but the test substance would be considered as "enhancing" the shoot weight as the mass is actually higher than the control. This is unlikely, but more likely due to natural biological variability. Even at 23.4 mg/kg there is essentially no effect, which attests to the variability of this biological parameter. The dose-response curve is very irregular for this species and parameter and the results are discussed qualitatively in the hazard assessment. It is concluded that toxicity in terrestrial plants following exposure to the read-across substance is unlikely.