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EC number: 288-315-1 | CAS number: 85711-55-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance is poorly soluble in water. At test initiation, nominal concentrations of the test substance were established by the addition of weights of the test substance to the respective vessel followed by the addition of RO (reverse osmosis) water (284 mL). Additions of synthetic sewage (16 mL) and microbial inoculums (200 mL) were made to give a final volume of 500 mL.
- Controls: Two controls without test material were tested in each test series, one at the start and one at the end. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: A sample of activated sludge was obtained the day before the start of the test. The mixed liquor suspended solids (MLSS) content was determined, synthetic sewage (50 mL/L) was added and the mixture was aerated overnight.
- Pretreatment: none
- Initial biomass concentration: ca. 4.0 g MLSS/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20-21 °C
- pH:
- test start: 7.4-7.5
test end: 8.0 - Dissolved oxygen:
- 6.0-7.6 mg O2/L (initial DO concentration in the cultures)
- Nominal and measured concentrations:
- 10, 30, 100, 300, 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers (test bottles) and biological oxygen demand (BOD) bottles
- Aeration: oil-free compressed air at 1 L/min
- No. of vessels per concentration (replicates): 10-300 mg/L: 1; 1000 mg/L: 3
- No. of vessels per control (replicates): 2
- Biomass loading rate: 1.6 g/L (MLSS)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reverse osmosis (RO) water
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen concentration after 3 hours contact time - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (3 h): 6.9 mg/L (95% confidence limits, 5.3 and 8.8 mg/L) - Reported statistics and error estimates:
- Since the permitted maximum difference for specific respiration rates in control beakers established at the beginning and end of the test period was 15%, this value was used to define the criteria for biologically significant levels of inhibition in mixtures containing the test substance.
The EC50 of the reference substance and 95% confidence limits were calculated using the SAFEstat curvefit programme (SAS Institute, 2002). - Validity criteria fulfilled:
- yes
- Remarks:
- Variations in respiration rates of controls <15%. The EC50 of 3,5-dichlorophenol was in the accepted range of 5-30 mg/L.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- conducted in accredited testing laboratory however without QA-statement
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: water accommodated fraction (WAF)
A saturated stock solution of the test material with an initial weight of 250 mg was prepared in 500 ml of water for injection and stirred overnight at 20 °C to 25 °C. After a filtration step over a sterile membrane filter (Minisart NML, Sartorius, Göttingen, Germany, pore size 0.2 µm) the solution was used in the test.
- Controls: water for injection - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: Pseudomonas putida DSM 50026, Stamm Berlin 33/2 obtained from the German collection of microorganisms (DSM, 38124 Braunschweig, Germany)
- Method of cultivation: Stock cultures were stored in a freezer at -80 °C in test tubes with Casein soya bean digest broth containing 7.5 % DMSO. The working culture of the strain Pseudomonas putida was stored in test tubes in solid nutrient medium. For the preservation of the test strain new strain cultures were made at one week intervals. These cultures were incubated and stored at 25 °C.
- Preparation of inoculum for exposure: The material of inoculation for the precultures was taken from a working culture up to 7 days old. One day prior to the start of the experiment the inoculation was multiplied in liquid medium. This grown preculture was diluted with medium in a way that a defined turbidity of FAU (Formazine Attenuation Units) = 10 was yielded arithmetically, e. g. by addition of 10 ml of a bacterial suspension with a turbidity of FAU = 100 to 90 ml of medium. The preculture was incubated for 7 hours at 21 °C ± 1 °C. After the incubation period the bacterial suspension was diluted with culture medium so that a defined turbidity of FAU = 50 was yielded arithmetically.
- Initial biomass concentration: FAU = 5 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 16 h
- Test temperature:
- 21°C +/- 1 °C
- Nominal and measured concentrations:
- nominal loading rate: 400 mg/L
DOC-value of the test solution at test start: 5.3 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flasks, fill volume 100 mL
- Bacterial starting concentration: FAU = 5
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: nutrient solution containing dextrose (2 g/L)
- Culture medium different from test medium: The culture medium contained supplemental yeast extract (50 mg/L)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): growth inhibition measured at test end - Reference substance (positive control):
- no
- Key result
- Duration:
- 16 h
- Dose descriptor:
- IC50
- Effect conc.:
- >= 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: results are expressed in terms of loading rates
- Duration:
- 16 h
- Dose descriptor:
- IC10
- Effect conc.:
- >= 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: results are expressed in terms of loading rates
- Details on results:
- In a saturated solution of the test material (a nominal loading rate 400 mg/L) no significant inhibition effects on the test organisms were observed.
- Validity criteria fulfilled:
- not specified
Referenceopen allclose all
Respiration rates were decreased by, at most, 1% in one mixture at a concentration of 1000 mg/L.
Table 1: Formazine Attenuation Units (FAU) at test end (16 ± 1 h)
Nominal loading rate (mg/l) |
Formazine Attenuation Units (FAU/436 nm) |
Inhibition (%) |
|||
Replicate |
Mean value |
||||
1 |
2 |
3 |
|||
400 |
342 |
348 |
362 |
351 |
4.7 |
Control cultures |
366 |
369 |
377 |
368 |
0 |
363 |
367 |
starting value of the biomass in the control (t0): 8
Description of key information
Toxicity to microorganisms: not toxic to microorganisms: EC10 >1000 mg/L (OECD 209 EU C.11); EC10 > 400 mg/L (DIN 38412, Pseudomonas putida test)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The results indicate that the test material is not toxic to sewage bacteria up to the limits of its water solubility and a NOEC and thus a PNEC cannot be determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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