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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This animal study is not performed according to GLP, but the test parameters are well documented and scientifically acceptable.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Hungarian authority requirements: MÉM Agrokémiai Főosztály
Principles of method if other than guideline:
Methods in Toxicology ( 19709 Balazs, T. Blackwell Scientific Publ. Oxf.-Edinburgh, 49-81.
Zbinden, G. and Flury-Roversi, M. (1981) Archives of Toxicology , 47, 77-99.
Finney, D.J (1952) Probit Analysis. Cambridge, Univ. Press
GLP compliance:
no
Test type:
other: Hungarian authority requirements: MÉM Agrokémiai Főosztály

Test material

Constituent 1
Chemical structure
Reference substance name:
EPTC
EC Number:
212-073-8
EC Name:
EPTC
Cas Number:
759-94-4
Molecular formula:
C9H19NOS
IUPAC Name:
N,N-dipropyl(ethylsulfanyl)formamide
Test material form:
not specified

Test animals

Species:
rat
Strain:
other: PVG/C, SPF
Sex:
male

Administration / exposure

Route of administration:
other: intraperitonealis szabad hasüregbe történő bejuttatás
Vehicle:
not specified
Doses:
Dózis ( mg / kg): 0, 475, 570, 665, 760, 808, 855
No. of animals per sex per dose:
4 male / dose
Control animals:
yes

Results and discussion

Preliminary study:
Doses: 23.7, 47.5, 71.2, 95.0, 118.8, 142.5, 237.5, 475.0, 950.0 -died a female, 1900.0 -died both , 3800.0 - died both
2 animals ( male and female)/doses
ALD = 950 mg/ bw-kg
Acute toxicity study is needed.
Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 760 mg/kg bw
Based on:
not specified
95% CL:
ca. 656 - 874
Mortality:
At 0 mg/kg dose 0 died out of 4 rats.
At 475 mg/kg dose 1 died out of 4
At 570 mg/kg dose 2 died out of 4
At 665 mg/kg dose 2 died out of 4
At 760mg/kg dose 3 died out of 4
At 808 mg/kg dose 4 died out of 4
At 855 mg/kg dose 4 died out of 4
Clinical signs:
Status: Néhány óra alatt kialakuló toxikus tünetek követik a parenteralis bevitelt. Rendkívüli levertség, tónustalanság, időnkénti görcsös rángások jellemzőek. Az elhullások 12-24 órán belül következnek be. A túlélő állatok állapota rendkívül lassan rendeződött.
Gross pathology:
Bonclelet: Az akut stádiumban elhullott állatok mellűri statusa negatív, az abdominalis status az irritalt peritoneum jellegzetes képét mutatja. Belövelt abdominalis zsigerek, erezett, lobos peritoneum, kismértékben megnagyobbodott máj. A túlélő állatokban az irritáció következtében peritonitis alakul ki, amely a szokásos képpel, - ascites, összenövések- járt.

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category IV
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
LD50 760 mg/ bw-kg this is acut toxicity category IV (between the cat IV limit 300-2000) Acut tox Category 4 H302
Executive summary:

According to this study the LD50 of EPTC is 760 mg/bw-kg. ( rat, male) Acut tox Category 4 H302