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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1977
Report date:
1977

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
no data
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
EPTC
EC Number:
212-073-8
EC Name:
EPTC
Cas Number:
759-94-4
Molecular formula:
C9H19NOS
IUPAC Name:
N,N-dipropyl(ethylsulfanyl)formamide
Details on test material:
The test compound was examined for mutagenic activity in a series of in vitro microbial assays employing Salmonella and Saccharomyces
indicator organisms. The compound was tested dlrectly and in the presence of llver microsomal enzyme preparations from Aroc]or-induced
rats.

Method

Target gene:
rat liver activatlon system and metabolic system
Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Test concentrations with justification for top dose:
10 to 5000 microgram/plate
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
not specified

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'. Remarks: no data

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test compound, Eptam Tech 3905-35, did not demonstrate mutagenic activity in the activation and nonactivation assays
conducted ]n this evaluation.
Executive summary:

The test compound, Eptam Tech 3905-35, did not demonstrate mutagenic activity in the activation and nonactivation assays

conducted in this evaluation.