Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Aug - Oct 1981
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Performed by an experienced lab using the commercial product according to guidelines in effect at the time.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1981

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: In accordance with the "Law Concerning the Examination and Regulation of Manufacture, etc., of Chemical Substances (Japan. 1973. Law No. 117.)
Principles of method if other than guideline:
14 d ready biodegradation test using high test material concentration and low innoculum concentration
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: crystalline
Details on test material:
light yellow crystalline powder, 99% purity, identified by infrared specturm

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Standard activated sludge 30 ppm (w/v)
Duration of test (contact time):
14 d
Initial test substance concentration
Initial conc.:
100 other: ppm (w/v)
Based on:
test mat.
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
test mat. analysis
Remarks:
UV after extraction with THF
Details on study design:
test temperature = 25 +/- 1 degree C
Apparatus: automated closed-system oxygen conusmpitn measuring apparatus (BOD-meter)
test vessel + basal culture medium + test material (100 ppm w/v)
test vessel + basal culture medium
test vessel + water + 100 ppm test material (100 ppm w/v)
test vesel + basal culture medium + aniline (100 ppm w/v)
Reference substance
Reference substance:
aniline
Remarks:
test invalid if O2 consumption does does not exceed 40% by 7 d

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
14 d
Parameter:
% degradation (test mat. analysis)
Value:
0
Sampling time:
14 d

Any other information on results incl. tables

% degradation of aniline after 7 d = 67

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
EBTBP was not readily biodegradable when tested in aerobic sludge at a high test material to sludge ratio for a duration of 14 d.
Executive summary:

EBTBP was not readily biodegradable when tested in aerobic sludge at a high test material to sludge ratio for a duration of 14 d.