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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Study on behalf of competent authorities of Japan,
equivalent or similar to guideline
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
not specified
Specific details on test material used for the study:
Purity: 98.5%
Lot: DU01
Code: K-1026
Oxygen conditions:
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:

liquid volume: 300 mL
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): derived from a mixture of source
Fushikogawa Treatment Plant (Sapporo City, Hokkaido)
Nakahama Treatment Plant (Osaka City, Osaka Prefecture)
Kitakami River (Ishinomaki City, Miyagi Prefecture)
Yoshino River (Tokushima City, Tokushima Prefecture)
Hiroshima Bay (Hiroshima City, Hiroshima Prefecture)
Fukashiba Treatment Plant (Kashima-gun, Ibaraki)
Ochiai Treatment Plant (Shinjuku-ku, Tokyo)
Shinano River (Nishikanbara-gun, Niigata)
Lake Biwa (Otsu City, Shiga Prefecture)
Dokai Bay (Kitakyushu City, Fukuoka Prefecture)

- Method of cultivation: The filtrate of each of the sludge collected in the above-mentioned regions was mixed with 54 filtrate of the old activated sludge that had been used for the test. The pH was adjusted to 7.0 ± 1.0 and aeration in an incubator
- Water filtered: yes
- The concentration of suspended substance in the activated sludge was 5800 mg/L
- pH adjusted to 7.0
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
other: HPLC
Reference substance:
other: HPLC
Sampling time:
4 wk
% degradation (TOC removal)
Sampling time:
4 wk
Remarks on result:
other: 3 - 4 %
% degradation (O2 consumption)
Sampling time:
4 wk
Remarks on result:
other: 1 - 5 %
Validity criteria fulfilled:
Interpretation of results:
not readily biodegradable
The test substance was not degraded by microorganisms under the test conditions.
Executive summary:

The test substance 3,5-xylidine was assessed in a biodegaradtion study according to OECD Guildeine 301C (modified MITI Test (I)).

The test substance was incubated in activated sludge for 28 days at a concentration of 100 mg/L with a liquid volume of 300 mL at 25 +/- 1°C. The measurement of the biochemical oxygen demand (BOD) was performed by a closed oxgen consumption measurment device and the quantitative analysis was performed by the analysis method of total organic carbon (TOC). Quantitative analysis of the test substance was done by high-preformance liquid chromatography (HPLC).

The test results were as follows:


BOD degradation rate: 1%, 5%, 0%, 

TOC degradation rate: 4%, 3%, 3%

Test substance degradation (HPLC): 0%, 0%, 0%, 


The tes substance was not degraded by microorganisms under the conditions of the test.

Description of key information

OECD 301 C, BOD degradation rate: 1%, 5%, 0%; TOC degradation rate: 4%, 3%, 3%; HPLC: 0%, 0%, 0% after 28 days


Key value for chemical safety assessment

Biodegradation in water:
not biodegradable
Type of water:

Additional information