(2-methoxyethyl)benzene

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental result using OECD guidelines.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Principles of method if other than guideline:

Biodegradation study was conducted according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test) for evaluating the percentage biodegradability of test chemical.

GLP compliance:

no

Oxygen conditions:

not specified

Inoculum or test system:

other: Mixed inoculum

Details on inoculum:

Mixed Inoculum Preparation:Polyseed were used for this study. 1 polyseed capsule was added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.

Duration of test (contact time):

42 d

Initial conc.:

4 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: OECD mineral medium was used for the study
- Test temperature: 20°C
- Continuous darkness: Yes
- Other: The water used in this study is deionized water.

TEST SYSTEM
- Culturing apparatus: The apparatus used in this study is BOD bottles; with glass stoppers (125 ml), BOD incubator & oxygen electrode and meter.

CONTROL AND BLANK SYSTEM
- Inoculum blank: it contains only test inoculum
- Procedure control: contains reference compound and inoculum

Reference substance:

other: Sodium Benzoate

Key result

Parameter:

% degradation (O2 consumption)

Value:

45.18

Sampling time:

42 d

Remarks on result:

other: Other details not known

Details on results:

The oxygen consumed by the test systems was corrected for oxygen consumption occurring in the blank test systems.The BOD Values (mgO2/mg) and percent biodegradation results for each test system are reported in Tables 2 and 3, respectively. The BOD42 value of test chemical was observed to be 1.23 mgO2/mg. ThOD was determined by calculation as 2.7 mgO2/mg. % degradation was calculated using the values of BOD and ThOD for test item and was determined to be 45.18% at 20 ± 1°C. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 78.31%. Degradation of Sodium Benzoate exceeds 42.16% on 7 days & 63.25% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid.

Results with reference substance:

The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 78.31%. Degradation of Sodium Benzoate exceeds 42.16% on 7 days & 63.25% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid.

TABLE 1: D.O Values (mg/L)

 

No. of Days	Inoculum Blank (Control)	Test Suspension	Procedure Control (Reference Item)
0	7	6.7	7
7	6.8	4.5	4
14	6.5	1.5	2.3
21	6.2	1	1.5
28	5.9	0.6	0.6
35	5.8	0.4	0.3
42	5.4	0.2	0.2

TABLE 2: BOD Values (mg O2/mg)

 

No. of Days	Test Suspension	Procedure Control (Reference Item)
0	0	0
7	0.5	0.7
14	1.17	1.05
21	1.22	1.17
28	1.25	1.32
35	1.27	1.37
42	1.22	1.3

 

TABLE 3: PERCENT BIODEGRDATION RESULTS

No. of Days	Test Suspension	Procedure Control (Reference Item)
0	0%	0%
7	18.51%	42.16%
14	43.33%	63.25%
21	45.18%	70.48%
28	46.29%	79.51%
35	47.03%	82.53%
42	45.18%	78.31%

 

Table 4: BOD42, THOD AND % BIODEGRADATION VALUES

 

Method details	BOD42(mgO2/mg)	ThOD (mgO2/mg)	%Biodegradation
Test Item	1.23	2.7	45.18
Reference Item	1.30	1.66	78.31

Validity criteria fulfilled:

yes

Interpretation of results:

other: ultimate inherently biodegradable

Conclusions:

The test chemical undergoes 45.18% biodegradation after 42 days in the test condition. Thus, the test chemical was considered to be ultimate inherently biodegradable in nature.

Executive summary:

42-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test chemical. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l . OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 78.31%. Degradation of Sodium Benzoate exceeds 42.16% on 7 days & 63.25% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid.The BOD42 value of test chemical was observed to be 1.22 mgO2/mg. ThOD was calculated as 2.70 mgO2/mg. Accordingly, the % degradation of the test item after 42 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 45.98%. Based on the results, the test item, under the test conditions, was considered to be ultimate inherently biodegradable in nature.

Description of key information

1: The test chemical undergoes 45.18% biodegradation after 42 days in the test condition. Thus, the test chemical was considered to be ultimate inherently biodegradable in nature.

WoE 2: Biodegradation study was conducted for 14 days to calculate the BOD, TOC and % degradation of test material during exposure period. Activated sludge of 30 mg/L conc was taken as inoculum and initial concentration of the test material was 100 mg/L. Test material analysis was done by GC. % degardation based on BOD was found to be 54.6. hence it can be concluded that the test item is not readily biodegradable.

WoE 3: Biodegradation study was conducted for 14 days to calculate the BOD, TOC and % degradation of test material during exposure period. Activated sludge of 30 mg/L conc was taken as inoculum and initial concentration of the test material was 100 mg/L. Test material analysis was done by GC. % degardation based on BOD was found to be 56. hence it can be concluded that the test item is not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Type of water:

freshwater

Additional information

1: 42-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test chemical (Experimental study report, 2018). The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 78.31%. Degradation of Sodium Benzoate exceeds 42.16% on 7 days & 63.25% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid. The BOD42 value of test chemical was observed to be 1.22 mgO2/mg. ThOD was calculated as 2.70 mgO2/mg. Accordingly, the % degradation of the test item after 42 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 45.98%. Based on the results, the test item, under the test conditions, was considered to be ultimate inherently biodegradable in nature.

WoE 2: Biodegradation study was conducted for 14 days to calculate the BOD, TOC and % degradation of test material during exposure period. Activated sludge of 30 mg/L conc was taken as inoculum and initial concentration of the test material was 100 mg/L. Test material analysis was done by GC. % degardation based on BOD was found to be 54.6. hence it can be concluded that the test item is not readily biodegradable.

WoE 3: Biodegradation study was conducted for 14 days to calculate the BOD, TOC and % degradation of test material during exposure period. Activated sludge of 30 mg/L conc was taken as inoculum and initial concentration of the test material was 100 mg/L. Test material analysis was done by GC. % degardation based on BOD was found to be 56. hence it can be concluded that the test item is not readily biodegradable.