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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The 48-Hour EL50 was determined to be 75 mg/L nominal loading rate and the No Observed Effect Loading rate was 56 mg/l nominal loading rate WAF. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
75 mg/L

Additional information

Introduction.A study was performed to assess the acute toxicity of the test material toDaphnia magna. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 202, “Daphnia sp. Acute Immobilisation Test and Reproduction Test” referenced as Method C.2 of Commission Directive 92/69/EEC.

Methods.Following a preliminary range finding study, twenty daphnids (2 replicates of 10 animals) were exposed to Water Accommodated Fractions of the test material over a range of nominal loading rates of 10, 18, 32, 56, and 100 mg/L for 48 hours at a temperature of 21 degrees C under static test conditions. The number of immobilized Daphnia were recorded after 24 and 48 hours.


Results.The 48 hour EL50 for the test material to Daphnia magna based on nominal loading rates was 75 mg/L WAF with 95% confidence limits of 56 to 100 mg/L. The 48 hour No Observed Effect loading rate was 56 mg/L loading rate WAF.