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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

The inhibition of the degradation activity of activated sludge  (EC50 1650 mg/L) is not anticipated.

Key value for chemical safety assessment

EC50 for microorganisms:
1 650 mg/L

Additional information

Introduction.A study was performed to assess the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984), No. 209 "Activated Sludge, Respiration Inhibition Test".

Methods.Activated sewage sludge was exposed to directly added test material at concentrations of 1, 10, 100, 1000 and 10000 ppm, a duplicate control group, and an assessment of the sensitivity of the inoculum used in the test to a reference toxicant 3,5-dichlorophenol. The test duration was a 3 hour exposure period at 20 degrees C to the test material followed by measurement of oxygen consumption.


Results.The effect of the test material on the respiration of activated sewage sludge gave an EC50 of 1,650 mg/L. The reference material gave an EC50 value of 12.5 mg/L. The activated sludge respiration test passed the quality control criteria for an acceptable test. The two control replicates produced oxygen consumption rates within the required 15% of each other.