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Toxicological information

Repeated dose toxicity: oral

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Administrative data

Endpoint:
repeated dose toxicity: oral
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: No GLP and no guideline was followed. Only the liver was examined for effects.
Cross-reference
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1982

Materials and methods

Principles of method if other than guideline:
The Wist 30 young rats (from Biobaza IISP, Cluj-Napoca, Romania), of both sexes, weighing 70-150 g, divided into 3 groupswere used. Control group, consisting 10 rats (5 males and 5 females) and tested groups consisting 10 rats (5 males and 5 females). One tested group was treated with 1% DL50 concentration dichloroacetyl chloride and the other with 5% DL50 concentration dichloroacetyl chloride. active. Dose was administrated at the beginning of the experiment and then twice a week. The conditions of care, feeding and administration were the same in' all the groups.The animals were monitored to fully consume the mixture received. On the 28th day of experiment animals were killed after deep anesthesia with ether and then, after laparotomy, the liver was harvested from which we sampled fragments for histological processing.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Dichloroacetyl chloride
EC Number:
201-199-9
EC Name:
Dichloroacetyl chloride
Cas Number:
79-36-7
Molecular formula:
C2HCl3O
IUPAC Name:
2,2-dichloroacetyl chloride
Details on test material:
- Name of test material (as cited in study report): dichloroacetyl chloride

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 70-150g

Administration / exposure

Route of administration:
other: oral with milk
Vehicle:
other: sunflower oil
Duration of treatment / exposure:
4 weeks
Frequency of treatment:
daily
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
1 % of LD50/d
Basis:
no data
Remarks:
Doses / Concentrations:
5%of LD50/d
Basis:
no data
No. of animals per sex per dose:
5 per sex
Control animals:
yes

Results and discussion

Results of examinations

Details on results:
In kidney and liver, no compound-related histopathological changes were evident
Kidney as well as liver: no substance-related histopathological effects were observed

Target system / organ toxicity

Critical effects observed:
not specified

Applicant's summary and conclusion

Conclusions:
Dichloroacetyl chloride does not show any toxic effect on the liver as far as the above concentrations and methodology are concerned.
Executive summary:

The Wist 30 young rats (from Biobaza IISP, Cluj-Napoca, Romania), of both sexes, weighing 70-150 g, divided into 3 groupswere used. Control group, consisting 10 rats (5 males and 5 females) and tested groups consisting 10 rats (5 males and 5 females). One tested group was treated with 1% DL50 concentration dichloroacetyl chloride and the other with 5% DL50 concentration dichloroacetyl chloride (dose repeated twice per week).

In conclusion, dichloroacetyl chloride does not show any toxic effect on the liver as far as the above concentrations and methodology are concerned.