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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Pre-GLP, pre-guideline study, which is to a great extent according to principles similar to those described in OECD TG 401

Data source

Reference
Reference Type:
publication
Title:
Food flavorings and compounds of related structure. I. Acute oral toxicity
Author:
Jenner PM, Hagan EC, Taylor JM, Cook EL, Fitzhugh OG
Year:
1964
Bibliographic source:
Food and Cosmetics Toxicology 2(3), 327-343

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
Groups of 10 young adult rats evenly diivided by sex were fasted for approximately 18 h prior to treatment. Animals had access to water ad libitum, and the food was replaced in cages as soon as animals received their respective doses. All doses were given by intubation. The usual observation period was 2 weeks. LD50 were computed by the method of Litchfield & Wilcoxon (1949).
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Allyl 3-cyclohexylpropionate
EC Number:
220-292-5
EC Name:
Allyl 3-cyclohexylpropionate
Cas Number:
2705-87-5
Molecular formula:
C12H20O2
IUPAC Name:
allyl 3-cyclohexylpropanoate
Details on test material:
- Name of test material (as cited in study report): Allyl cyclohexylpropionate
- Analytical purity: no data

Test animals

Species:
rat
Strain:
Osborne-Mendel
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS

- Fasting period before study: 18 h
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Details on oral exposure:
MAXIMUM DOSE VOLUME APPLIED: no data
Doses:
no data
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: close observation until animals appeared normal and showed weight gain.
- Other examinations performed: clinical signs
Statistics:
LD50 was computed by the method of Litchfield & Wilcoxon (1949).

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
585 mg/kg bw
Based on:
test mat.
95% CL:
480 - 714
Mortality:
the death was recorded starting at 4 h and on day 6 post-dose
Clinical signs:
Depression, rough fur
Body weight:
no data
Gross pathology:
no data
Other findings:
no data

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category IV
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The substance was acutely toxic to rats in an acute oral toxicity test with a LD50 value of 585 mg/kg bw. The substance is classified into Acute Toxicity Category IV according to CLP.
Executive summary:

The acute oral (gavage) toxicity of the allyl 3-cyclohexylpropionate was studied in a non-GLP test in male and female Osborne-Mendel rats according to principles generally similar to those of OECD TG 401. 5 animals per sex per dose were exposed to single oral (gavage) doses (dose levels were not reported). Animals were observed during a period of 14 days. Time of death was recorded between 4 h and 6 days post-dose. The LD50 value was 585 mg/kg/day with a 95% confidence interval of 480 and 714 mg/kg/bw. Depression and rough fur were observed as toxic signs.