3-[[5-methyl-2-(1-methylethyl)cyclohexyl]oxy]propane-1,2-diol

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 July 1984 to 24 October 1984

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: "Bioaccumulation test of chemical substances in fish and sellfish" described in "Kanpogyou, No.5", "Yakuhatsu, No.615" and "49 Kikyoku, No.392

GLP compliance:

yes

Specific details on test material used for the study:

- Appearance under ambient temperature; transparent clear liquid

Solubility;
Water: more than 500 mg/L (by TOC meter)
n-Hexane: more than 1000 mg/L
Chloroform: more than 1000 mg/L
Benzene: more than 1000 mg/L
Acetonitrile: more than 1000 mg/L

Radiolabelling:

no

Details on sampling:

(1) Test water;
The test substance in the test water was analysed twice every week, 16 times in total for both concentration 1 and 2 area during exposure period using one sample each time.
(2) Test fish;
The test substance in the test fish was analysed 2,4,6 and 8 week, 4 times in total, after the initiation of exposure for both concentration 1 and 2 area, using two fish samples each time.

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

(1) Test material concentration of test water
The test material concentration in the test aquarium was decided as follows
Concentration 1 area: 0.5 mg/L (ppm)
Concentration 2 area; 0.05 mg/L (ppm)
(2) Preparation method of stock solution
The test substance (1.5 g) and 5 times amount of HCO-40 (polyoxyethylene hardened castor oil derivative; NIKKO CHEMICALS CO., LTD.) were dissolved in acetone and mixed. Then, the acetone was distilled away and added deionized water. The mixture was stirred to obtain suspended stable solution of 5000 mg/L concentration. This solution was diluted with deionised water to obtain each stock solution of 25 L for concentration 1 area (50 mg/L) and concentration 2 area (5 mg/L). The each stock solutions were prepared twice a week during exposure period.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST FISH;
Common name: Common carp
Species: Carp (Cyprinus carpio)
Source: Kitamura fish farm (Yatsushiro-city, Kumamoto)
Lot. No.: TFC840717
Medical bath: The medical bath was performed using tetracycline hydrochloride solution (10 mg/L) under static water conditions during 24-hours.
Acclimatisation period: 14 days, 25°C.
Standard size of used fish*:
Mean body weight; 22.4 g
Mean length; 9.7 cm
Mean content of lipid; 4.2%
* Measured value of representative sample from same acclimatisation lot.

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Hardness:

no description

Test temperature:

Breeding temperature: 25 ± 2°C

pH:

no description

Dissolved oxygen:

Dissolved oxygen concentration in test aquarium:
Concentration 1 area; 5.7 – 7.3 mg/L
Concentration 2 area; 6.2 – 7.5 mg/L

TOC:

no discription

Salinity:

no data due to freashwater study

Details on test conditions:

TEST SYSTEM;
(1) Breeding equipment: Environmental control equipment with a flow-through water system
(2) Breeding bath: 100 L glass aquarium
(3) Flow rate: 582 L/day
(stock solution : dilution water = 4 mL/minute : 400 mL/minute)
(4) Number of test fish used: 20 fish each breeding aquarium ( at the initiation of exposure)
(5) Breeding period: 8 weeks
(6) Breeding temperature: 25 ± 2°C
(7) Feeding: An amount of corresponding to about 2% of the test fish bodyweight was fed twice daily in halves.

RATIONAL OF THE TEST MATERIAL CONCENTRATION FOR DEFINITIVE TEST;
Based on the preliminary test result for 48-hr LC50, the test material concentrations for definitive test were determined as follows;
Concentration 1 area: 0.5 mg/L
Concentration 2 area: 0.05 mg/L

PRELIMINARY TEST;
(1) Test method: The preliminary test was performed according to the method of “Acute Toxicity Test in Fish” described in Factory Effluent Test Method provided in JIS K 0102 using HCO-40 as dispersing agent.
(2) Species: Killifish (Oryzia latipes)
(3) During exposure: 48 hours
(4) Test result: 48-hr LC50 of test substance; 48.5 mg/L
(5) Dilution water: well water

Nominal and measured concentrations:

The nominal test material concentrations and the mean measured test material concentrations of test water were shown in Table.2.

Reference substance (positive control):

no

Lipid content:

4.2 %

Time point:

start of exposure

Remarks on result:

other: The values of mean body weight, mean length and mean content of lipid for used fish were shown measured values of reperasentative samplefrom same acclimatisation lot.

Conc. / dose:

0.5 mg/L

Temp.:

25 °C

Type:

BCF

Value:

>= 2.1 - <= 3.3 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: no data on pH provided

Conc. / dose:

0.05 mg/L

Temp.:

25 °C

Type:

BCF

Value:

>= 2.1 - <= 3.9 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: no data on pH provided

Details on kinetic parameters:

no description

Metabolites:

no description

Results with reference substance (positive control):

no use of reference substance

Details on results:

Abnormalities of test fish; No macroscopic abnormalities of test fish were observed during test period.
Mean test material concentration of test water;
As the mean test material concentration of test water, the measured values more than 90% of nominal concentration shown in Table 2 were maintained during exposure period.
Bioaccumulation factor; Table 1

Table 1: Bioaccumulation factor

Table 2: Test material concentration of test water (nominal and measure value)

Validity criteria fulfilled:

not specified

Conclusions:

The bioconcentration factor of the test material was determined to be between 2.1 and 3.9 over a period of 8 weeks.

Executive summary:

The bioaccumulation potential of the test material was determined in a study conducted to the method "Bioaccumulation test of chemical substances in fish and shellfish" described in "Kanpogyou, No.5", "Yakuhatsu, No.615" and "49 Kikyoku, No.392 under conditions of Good Laboratory Practice.

The bioconcentration factor of the test material was determined over a period of 8 weeks to be between 2.1 and 3.3 when the test material was present in nominal concentrations of 0.5 mg/L and between 2.1 and 3.9 when fish were exposed to concentrations of 0.05 mg/L.

Description of key information

The bioconcentration factor of the test material was determined to be between 2.1 and 3.9 over a period of 8 weeks.

Additional information

The bioaccumulation potential of the test material was determined in a study conducted to the method "Bioaccumulation test of chemical substances in fish and shellfish" described in "Kanpogyou, No.5", "Yakuhatsu, No.615" and "49 Kikyoku, No.392 under conditions of Good Laboratory Practice. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).

The bioconcentration factor of the test material was determined over a period of 8 weeks to be between 2.1 and 3.3 when the test material was present in nominal concentrations of 0.5mg/L and between 2.1 and 3.9 when fish were exposed to concentrations of 0.05 mg/L.