Trisodium 7-(4-(4-fluoro-6-(2-(2-vinylsulfonylethoxy)ethylamino)-1,3,5-triazine- 2-ylamino)-2-ureidophenylazo)naphthalene-1,3,6-trisulfonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 February 1987 to 26 February 1987

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EEC Directive 84/449, Part C ."Acute Toxicity for Daphnia", September 19, 1984.

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: OP. 1/86 VERS. 10/86
- Expiration date of the lot/batch: 01 October 1991

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: AT room temperature protected from light and humidity
- Stability under storage conditions: Stable in water and organic solvents for atleast 2 h
- Solubility of the test substance in the solvent/dispersant/vehicle/test medium: Aqueous: >50 g/l at 20 °C

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

FAT 40'224/C was dissolved in the test medium at a concentration of 100 mg/100 ml (stock dilution). Thereafter, series of sequential dilutions with the test medium were prepared to obtain the final test concentrations of the test article. Along with the test article, control samples were run.

Test organisms (species):

Daphnia magna

Details on test organisms:

The toxicity study was run using the species Daphnia magna. The animals were bred in the laboratories of RCC under standardized conditions. For the tests, animals with an age of less than 24 hours were used.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Hardness:

CaC12 x 2H20:294 mg/L
MgS04 x 7H20:123 mg/L
NaHC03: 65 mg/L

Test temperature:

20 ± 2 °C

pH:

7.7-8.1

Dissolved oxygen:

8.8-9.0 mg/L

Nominal and measured concentrations:

Nominal: 0, 62.5, 125, 250, 500 or 1000 mg/L

Details on test conditions:

The daphnids mere exposed to the test article in 50 mL beakers containing 20 mL test medium per 10 animals. During exposure, the animals were kept at 20 ± 2 °C with 16 hours of illumination. All tests were run in duplicate.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Based on the results presented, the following immobilization data were determined:
EC0: Exposure time 24 hours >1000 mg/L.
EC50: Exposure time 24 hours >1000 mg/L.
EC100: Exposure time 24 hours >1000 mg/L.

Results with reference substance (positive control):

Based on the given data, the EC50 estimate (Logit model) for a 24 hours exposure of daphnids to potassium dichromate was:
EC50 (24 hrs.) - 1.493 mg/L. The 95 % confidence limits for the EC50 value were: EC50 (24 hrs.): 1.382 - 1.606 mg/L.

Reported statistics and error estimates:

The EC 50 was estimated by using the Logit model.

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of FAT 40224/C was determined to be >1000 mg/L.

Executive summary:

Acute toxicity of the test substance to Daphnia magna was determined in a immobilization test performed according to the OECD guideline 202. Ten animals per dose were placed in a static system and exposed for 24 h to 0, 62.5, 125, 500 or 1000 mg/L of test substance. During exposure, the animals were kept at 20 ± 2 °C with 16 h of illumination and all tests were run in duplicate. No significant effect on mobilisation was noted. The 24 h EC50 of FAT 40224/C was determined to be >1000 mg/L. This leads to the conclusion that  FAT 40224 is considered to be nontoxic to daphnids.

Description of key information

Acute toxicity of the test substance to Daphnia magna was determined in a immobilization test performed according to the OECD guideline 202. Ten animals per dose were placed in a static system and exposed for 24 hours to 0, 62.5, 125, 500 or 1000 mg/L of test substance. During exposure, the animals were kept at 20 ± 2 °C with 16 hours of illumination and all tests were run in duplicate. No significant effect on mobilisation was noted. The 24 h EC50 of FAT 40224/C was determined to be >1000 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 000 mg/L