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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Samples were taken from all treatments at 0 and 24h.
Vehicle:
no
Details on test solutions:
A stock solution of 3g/L (based on the active component) is prepared by disolving 16.077g of test substance in 500 mL demineralised water.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: LISEC laboratory culture
- Age: less than 24 hours old at the start of the test
- Age of the parent daphnids: about three weeks old
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20.3 - 21.3°C
pH:
7.3 - 8.29
Dissolved oxygen:
>= 2.5 mg/L
Nominal and measured concentrations:
Nominal test concentrations based on the active component: 0, 6.25, 12.5, 25, 50 and 100 mg/L
Details on test conditions:
- Renewal of the test solutions: after 24 hours
- Test containers: 50 mL borosilicate glass beakers
- Volume of test solution: 50 mL per vessel
- Number of organisms: 20 daphnids are used for the blank and for each test concentration divided into four groups of 5 animals
- Feeding: the daphnids are not fed during the test
- Because of the photodegradation of the test substance under light, the test was carried out entirely in the dark
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Qualifier:
according to
Guideline:
other: USEPA 1975 Methods for acute toxicity tests with fish, macroinvertebrates and ampphibianes EPA-660/3-75-009
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: Penaeus duorarum
Details on test organisms:
TEST ORGANISM
- Common name: Penaeus duorarum, pink shrimp
- Source: Big Lagoon, FL
- Age at study initiation (mean and range, SD): juvenile shrimp
- Weight at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 20 - 30 mm
- Feeding during test: no

ACCLIMATION
- Acclimation period: 14
- Acclimation medium (same as test or not): the same as in the test, natural seawater
- Health during acclimation (any mortality observed): mortality was estimated to be < 3 %
Test type:
static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
22 +/- 1 °C
pH:
after 96 hours: 7.8
Dissolved oxygen:
after 96 hours: 2.8 mg/L (36 % of saturation)
Salinity:
20 °/oo
Nominal and measured concentrations:
Test concentrations nominal: control and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass jars
- Size, fill volume: 19 L, 15 L
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- natural seawater

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1000 ppm
- Results used to determine the conditions for the definitive study: yes
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 747 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
< 747 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.

Description of key information

With high probability acutely not harmful to aquatic invertebrates.

EC50 (48h) > 100 mg/L (nominal) for Daphnia magna (OECD 202)

LC50 (96h) > 747 mg/L (nominal) for Penaeus duorarum (salt water) (EPA-660/3-75-009)

Key value for chemical safety assessment

Additional information

The acute toxicity on aquatic invertebrates was investigated for ‘zinc phthalocyanine sulfonate’ with the waterflea Daphnia magna, in a GLP guideline study according to OECD 202 (Procter & Gamble 1994).  The test daphnia were exposed to the substance for 48 hours in a semi-static test system. At test termination an EC50 > 100 mg/L (nominal) was determined.

In a supporting stuy the toxicity of ‘zinc phtalocyanine sulfonate’ to aquatic invertebrates was investigated according to USEPA 1975 Methods for acute toxicity tests with fish, macroinvertebrates and amphibianes EPA-660/3-75-009 (Procter & Gamble 1978). In this study the pink shrimp Penaeus duorarum was exposed to the substance for 96 hours in a salt water limit test with a nominal concentration of 1000 mg/L (nominal) under static conditions. At test termination the LC50 > 747 mg/L (nominal) was determined.