Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study according to EU / OECd guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3,3-dimethylbutyryl chloride
EC Number:
230-356-4
EC Name:
3,3-dimethylbutyryl chloride
Cas Number:
7065-46-5
Molecular formula:
C6H11ClO
IUPAC Name:
3,3-dimethylbutyryl chloride

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
20 ± 2° C
pH:
7.6
Nominal and measured concentrations:
1000, 1800, 3200, 5600, 10000 mg/L
Reference substance (positive control):
yes
Remarks:
3.5-Dichlorophenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
>= 6 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
The test item showed 80.2 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test item showed 80.2 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L.
EC50 = 6120mg/L