Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

Key study: 48 h EC50 0.40 mg a.i./L, Daphnia magna, OECD 202, EPA OPPTS 850.1010, Broughter et al. 2013b

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.4 mg/L

Additional information

The acute toxicity of the test material to aquatic invertebrates was investigated in Daphnia magna in a GLP study which was conducted in accordance with standardised guidelines OECD 202 and EPA OPPTS 850:1010. During the study the Daphnia were exposed to the test material at nominal concentrations of 0, 0.043, 0.094, 0.21, 0.45 and 1.0 mg a.i./L under flow-through conditions for 48 hours. The mean measured concentrations of the test material were 0, 0.032, 0.060, 0.12, 0.31 and 0.71 mg a.i./L, representing 40 - 90 % of the nominal values. Daphnia in the negative and solvent control groups appeared normal throughout the test, with no immobility or signs of toxicity. Percent immobility at test termination in the 0.032, 0.060, 0.12, 0.31 and 0.71 mg a.i./L mean measured concentration treatment groups was 0, 0, 5, 20 and 100 %, respectively. Floating daphnids were observed in the 0.12, 0.31 and 0.71 mg a.i./L treatment groups at the initial observation approximately 4 hours after test initiation, and at the 24 hour observation interval in the 0.12 and 0.31 mg a.i./L treatment groups. All floating daphnids in the 0.12, 0.31 and 0.71 mg a.i./L treatment groups appeared normal after being gently submerged. Lethargy was also observed in the 0.12 and 0.31 a.i./L treatment groups at the final observation interval (48 hours). Under the conditions of the study the 48 hour EC50 of the test material based on mean measured test concentrations was determined to be 0.40 mg a.i./L with a 95% confidence interval of 0.31 to 0.71 mg a.i./L. The no-immobility concentration was 0.060 mg a.i./L and the No Observed Effect Concentration was 0.060 mg a.i./L.

The study was performed in line with GLP and accepted standardised guidelines with a high standard of reporting. The study was assigned a reliability score of 1 in accordance with the principles for assessing data quality as defined in Klimisch (1997).