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EC number: 309-713-4 | CAS number: 100684-40-0 A complex combination of hydrocarbons obtained as a residue from the distillation of crude oil under vacuum. It consists predominantly of hydrocarbons having carbon numbers predominantly in the range above C50 and boiling in the range above approximately 500°C (932°F).
A read across in vivo micronucleus test on oxidised bitumen was negative for genotoxicity. In addition chronic inhalation studies with oxidised bitumen, together with comparative fume compostion information, indicate that read across to the bitumen category, is appropriate.
In a mammalian micronuclei assay, Chinese hamster cell V79 cultures were exposed to Type I and Type III Asphalt Fume Condensates in DMSO at concentrations of 0, 62.5, 125, 187.5, and 250 μg/mL for 24 hours.
Cells were subcultured at a concentration of 5 x 105 cells/ 60 mm dish for each treatment. Incubation Period: Cells were incubated at 37 degrees Celsius in a 5% carbon dioxide humidified atmosphere for 24 hours. The asphalt fume condensate dissolved in DMSO was added to each culture. Four different concentrations were tested for each sample. Cells were treated for 24 hours, then washed twice with PBS, and fresh medium was placed in each disk. After an additional 24-hr incubation, cells were detached by trypsinization, collected by centrifugation, and resuspended in culture medium at a density of 1 x 106 cells/mL.
The results were expressed as the mean number of cells with micronucleus per 1000 cells. Both types of Asphalt Fume Condensates were found capable of causing micronucleaus formation in mammalian cells in vitro. The genotoxic potential appears to be similar for both types of asphalt condensate. Both condensates caused a similar dose-related increase in the frequency of micronucleated cells. The increase was statistically significant for all four concentrations tested. These findings indicate that both Type I and Type IIl roofing asphalt fumes are capable of causing principally cytogenetic damage by spindle apparatus in cultured mammalian cells.
This study received a Klimisch score of two and is classified as reliable with restrictions because it is an acceptable and a well-documented study report.
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