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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
yes
Principles of method if other than guideline:
The volume of the test solution was reduced from 3.0 1 to 1.5 1. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. The test substance was added directly into the medium without preparing a stock solution.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2-difluoro-benzo[1,3]dioxole-4-carbaldehyde
EC Number:
700-476-6
Cas Number:
119895-68-0
Molecular formula:
C8H4F2O3
IUPAC Name:
2,2-difluoro-benzo[1,3]dioxole-4-carbaldehyde

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
10.9 mg/L
Based on:
test mat.
Initial conc.:
21.6 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (CO2 evolution)
Value:
1
Sampling time:
28 d
Remarks on result:
other: test substance concentration 10.9 mg/l
Parameter:
% degradation (CO2 evolution)
Value:
0
Sampling time:
28 d
Remarks on result:
other: test substance concentration 21.6 mg/l

BOD5 / COD results

Results with reference substance:
87 % of the reference substance (Aniline) was degraded after 28 d

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The test substance is not readily biodegradable in the OECD301B test (CO2 Evolution Test).