2,2-difluoro-benzo[1,3]dioxole-4-carbaldehyde

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

(1984)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2-difluoro-2H-1,3-benzodioxole-4-carbaldehyde

EC Number:

700-476-6

Cas Number:

119895-68-0

Molecular formula:

C8H4F2O3

IUPAC Name:

2,2-difluoro-2H-1,3-benzodioxole-4-carbaldehyde

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Composite samples (about 150 ml in duplicate) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18OC to - 22OC until analysis.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

240 mg CaCO3 /l

Test temperature:

20°C +/-1°C

pH:

0h 48h
blank 8.1 7.9
5.5 mg/l 8.1 7.9
12.3 mg/l 8.1 7.9
23.3 mg/l 8.1 7.9
42.6 mg/l 8.1 7.9
50 mg/l 8.1 7.9

Dissolved oxygen:

0h 48h
blank 97% 98%
5.5 mg/l 97% 98%
12.3 mg/l 97% 98%
23.3 mg/l 97% 98%
42.6 mg/l 97% 98%
50 mg/l 97% 98%

Nominal and measured concentrations:

nominal measured initial (0h) measured end (48h)
blank <0.01 <0.01
10 mg/l 10.1 mg/ml 5.5 mg/l
18 mg/ml 19.5 mg/ml 12.3 mg/l
32 mg/ml 34.3 mg/ml 23.3 mg/l
58 mg/ml 59.5 mg/ml 42.6 mg/l
100 mg/ml 104.3 mg/ml 50 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers covered with watch glasses 100 ml solution per beaker
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4

OTHER TEST CONDITIONS

- Photoperiod: Fluorescent light, 16 hours daily
- Light intensity: approx. 1500 lux

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

22.1 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

12.3 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

42.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility