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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
(1984)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2-difluoro-benzo[1,3]dioxole-4-carbaldehyde
EC Number:
700-476-6
Cas Number:
119895-68-0
Molecular formula:
C8H4F2O3
IUPAC Name:
2,2-difluoro-benzo[1,3]dioxole-4-carbaldehyde

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Composite samples (about 150 ml in duplicate) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18OC to - 22OC until analysis.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
240 mg CaCO3 /l
Test temperature:
20°C +/-1°C
pH:
0h 48h
blank 8.1 7.9
5.5 mg/l 8.1 7.9
12.3 mg/l 8.1 7.9
23.3 mg/l 8.1 7.9
42.6 mg/l 8.1 7.9
50 mg/l 8.1 7.9
Dissolved oxygen:
0h 48h
blank 97% 98%
5.5 mg/l 97% 98%
12.3 mg/l 97% 98%
23.3 mg/l 97% 98%
42.6 mg/l 97% 98%
50 mg/l 97% 98%
Nominal and measured concentrations:
nominal measured initial (0h) measured end (48h)
blank <0.01 <0.01
10 mg/l 10.1 mg/ml 5.5 mg/l
18 mg/ml 19.5 mg/ml 12.3 mg/l
32 mg/ml 34.3 mg/ml 23.3 mg/l
58 mg/ml 59.5 mg/ml 42.6 mg/l
100 mg/ml 104.3 mg/ml 50 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Beakers covered with watch glasses 100 ml solution per beaker
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4

OTHER TEST CONDITIONS

- Photoperiod: Fluorescent light, 16 hours daily
- Light intensity: approx. 1500 lux

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
22.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
12.3 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
42.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion