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Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Version / remarks:
(1984)
Deviations:
yes
Principles of method if other than guideline:
Instead of a centrifuged sludge a settled sludge was used. Due to the poor solubility of the test substance at test concentrations, no stock solution was prepared. The test substance was given directly into the medium. Therefore, a constant factor between the test concentrations could not been achieved.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2-difluoro-benzo[1,3]dioxole-4-carbaldehyde
EC Number:
700-476-6
Cas Number:
119895-68-0
Molecular formula:
C8H4F2O3
IUPAC Name:
2,2-difluoro-benzo[1,3]dioxole-4-carbaldehyde

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 13/04/92. The pH after collection was 7.6.
The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation.

Study design

Water media type:
freshwater
Total exposure duration:
3 h

Test conditions

Test temperature:
20 +/- 2°C
pH:
8.2 - 8.4
Nominal and measured concentrations:
Test substance:
100.6, 43.0, 16.9, 6.6, 2.8, 1.2, mg/l

Reference substance:
32, 10, 3.2 mg/l
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC50 (3h): 13.6 mg/l

Any other information on results incl. tables

Sample

Conc. [mg/l]

Consumption rate [mg/l/h]

Inhibition [%]

pH

Blank 1

 0

47.6 

 -1

8.2 

Blank 2

 0

45.4

 2

8.2

Reference 1

 32

10.7 

 77

8.5

Reference 2

 10

27.5

 41

8.5

Reference 3

 3.2

44.6

 4

8.5

Test conc. 1

 100.6

47

 -1

8.2

Test conc. 2

 43

46.1

 1

8.3

Test conc. 3

 16.9

47.4

 -2

8.4

Test conc. 4

 6.6

47.3

 -2

8.4

Test conc. 5

 2.8

47.5

 -1

8.4

Test conc. 6

 1.2

47.6

 -2

8.4

Applicant's summary and conclusion