3-methyl-2-butenyl acetate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study, no preincubation test performed

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3-methyl-2-butenyl acetate

EC Number:

214-730-4

EC Name:

3-methyl-2-butenyl acetate

Cas Number:

1191-16-8

Molecular formula:

C7H12O2

IUPAC Name:

3-methylbut-2-en-1-yl acetate

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): PRENYL ACETATE
- Physical state: colourless to pale yellowish liquid
- Analytical purity: 98.7%
- Batch No.: 20010024
- Storage conditions: cool and dry

Method

Target gene:

histidine operon

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102

Metabolic activation:

with and without

Metabolic activation system:

S9 from SD male rats induced with Aroclor 1254

Test concentrations with justification for top dose:

0, 50, 150, 500, 1500 and 5000 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: Without S9-mix: 0.7 µg/plate sodium azide (NaN3; TA100, TA1535); 2.5 µg/plate 2-nitrofluorene (2-NF; TA98); 0.15 µg/plate mytomycin C (TA102); 50 µg/plate 9-aminoacridine (9-AA; TA1537). With S9-mix: 0.8-1.7 µg/plate 2-aminoanthracene (2-AA; all strains).

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

DURATION
- Exposure duration: 48 to 72 hours in the dark

NUMBER OF REPLICATIONS: triplicates; the whole experiment was repeated in full after at least 3 days

DETERMINATION OF CYTOTOXICITY, OTHER EXAMINATIONS:
background lawn and microcolony growth.

Evaluation criteria:

The test substance is considered positive if a significant or dose related increase in the mutation frequency of the tester strains in the absence and presence of a metabolic activation system is observed.

Statistics:

The statistical significance of the difference between the mean number of revertants in the negative controls and the plates at each dosage level was estimated using a X2-test.

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Additional information on results:

TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No precipitation was observed

The results with the positive control substances confirmed the known reversion properties and specificity of the tester strains as well as the full activity of the metabolizing system.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.