2-amino-2-ethylpropanediol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 Apr - 11 Jun 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The substance amount required for the production of the test concentration is weighed in a plastic cup and inserted into a weighted sample receptacle.

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: Golden ide
- Source: Fish Farm Eggers, Hohenweststedt, Germany
- Age at study initiation: ca. 4 weeks
- Corpulence factor (W(g) x 100/L(cm3)): 0.8 - 1.1 g/cm
- feeding during test: none

ACCLIMATION
- Type and amount of food: Pitti Floxi-Flakes, alround food for ornamental fish
- Feeding frequency: 2x weekly
- Lighting: 12 hours per day
- Oxygen content: at least 80 % of saturation value

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

15 ± 3 °dH

Test temperature:

20 ± 0.5 °C

pH:

8.3 - 9.3

Dissolved oxygen:

5.3 - 8.1 mg O2/L

Nominal and measured concentrations:

nominal: 100, 200, 400, 600, and 800 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: glass container (length: 28.5; width: 21.0; height: 24.0 cm)
- Aeration: lightly aerated during the test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A mix of Cologne tap water and deionised water serves as dilution water
- Ca/mg ratio: 4:1
- Intervals of water quality measurement: after 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12 hours per day

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : lethality, after 24, 48, 72, and 96 hours

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

460 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Table 3: mortalities during test

		Concentration (mg/L)
		control	100	200	400	600	800
24h	Absolut rel.%	0/10 0	0/10 0	0/10 0	2/10 20	2/10 20	5/10 50
48h	Absolut rel.%	0/10 0	0/10 0	0/10 0	2/10 20	5/10 50	9/10 90
72h	Absolut rel.%	0/10 0	0/10 0	0/10 0	2/10 20	7/10 70	9/10 90
96h	Absolut rel.%	0/10 0	0/10 0	0/10 0	2/10 20	8/10 80	10/10 100

Description of key information

LC50(96h) = 460 mg/L for Leucidus idus melanotus (OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

460 mg/L

Additional information

There is one short term fish study available for 2-amino-2-ethyl-1,3-propanediol (AEDP), which was used as key study for the assessment. The study was conducted according to OECD guideline 203 (Schopka, 1991). The test organism Leuciscus idus melanotus was exposed to AEPD in a static system for 96 hours, at the nominal test concentrations 100, 200, 400, 600, and 800 mg/L. As a result, an LC50 of 460 mg/L was obtained.