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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: scientific acceptable and sufficient documented

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Principles of method if other than guideline:
100 µl test substance was applied into the conjuctival sac of 2 rabbits. Post exposure time was 7 days. Eyes were examinated after application of test substance up to 7 days
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
tert-butyl isocyanate
EC Number:
216-544-9
EC Name:
tert-butyl isocyanate
Cas Number:
1609-86-5
Molecular formula:
C5H9NO
IUPAC Name:
2-isocyanato-2-methylpropane
Details on test material:
Test substance: tert-butyl isocyanate

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
undiluted
Amount applied: 100 other: µl
Observation period (in vivo):
7 days
Number of animals or in vitro replicates:
2

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
1.7
Max. score:
4
Reversibility:
not fully reversible within: 7 days
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
1.8
Max. score:
2
Reversibility:
not fully reversible within: 7 days
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
2.5
Max. score:
3
Reversibility:
not fully reversible within: 7 days
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
2.5
Max. score:
4
Reversibility:
not fully reversible within: 7 days

Any other information on results incl. tables

Immediately after application a moderate/strong redness and a moderate/very strong swelling were observed. Cornea was clouded and cauterized. All  findings persist up to end of observation period

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information
Executive summary:

100 µl test substance was applied into the conjuctival sac of 2 rabbits. Post exposure time was 7 days. Eyes were examinated after application of test substance up to 7 days.

Immediately after application a moderate/strong redness and a moderate/very strong swelling were observed. Cornea was clouded and cauterized. All  findings persist up to end of observation period. Tert-butyl isocyanate is strong irritant and corrosive to the cornea