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EC number: 300-339-7 | CAS number: 93925-37-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 September 1997 - 3 October 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed in accordance with guideline; restriction due to lack of analytical analyses of test solutions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: US. EPA (TSC) 797.1050
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-Propenoic acid, butyl ester, reaction products with butadiene, sulfur and tri-Ph phosphite
- EC Number:
- 300-339-7
- EC Name:
- 2-Propenoic acid, butyl ester, reaction products with butadiene, sulfur and tri-Ph phosphite
- Cas Number:
- 93925-37-2
- Molecular formula:
- C18H15O3P.C7H12O2.C4H6.S as an example
- IUPAC Name:
- 2-Propenoic acid, butyl ester, reaction products with butadiene, sulfur and tri-Ph phosphite
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 0, WAF (0.3, 3.3, 33, 330, and 3,300) mg/L
- Sampling method:
Cell counts were made on replicated samples and recorded at 72 and 96 hours.
At the conclusion of the test, a 0.5ml sample of test media from each 3,300 mg/L vessel was combined in a flask with 100 rnl of fresh media to determine whether toxic effects were algicidal or algistatic. The flask was incubated under test conditions for 216 hours and examined for the presence of algal cells.
- Sample storage conditions before analysis: not applicable.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Each of the five WAFS were prepared by combining the appropriate amount of test substance and dilution water in a mixing vessel equipped with a magnetic stirrer (the vortex extended form the surface approximately 25% of the way to the bottom of the mixing vessel) and stirring these mixtures for approximately 20 hours, settling the mixtures for approximately four hours, and siphoning the water phase containing the WAF.
- Eluate: Not applicable.
- Differential loading: Not applicable.
- Controls: Negative control
- Concentration of vehicle in test medium (stock solution and final test solution): Not applicable.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): the 3300 mg/L test solution was cloudy throughout the test.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Algae
- Strain: Scenedesmus subspicatus strain UTEX 1648.
- Source (laboratory, culture collection): from a culture originally procured from the Culture Collection of Algae at the hversity of Texas at Austin and delivered to T.R. Wilbury Laboratories on January 30, 1997.
- Age of inoculum (at test initiation): seven day- old culture
- Method of cultivation: The culture was transferred to sterile enriched media identical to media used for this test and maintained at test conditions for at least 14 days before the definitive test. The subsample of algae used to inoculate media at the start of the definitive test came from a seven day- old culture. Identification of the culture organisms was verified using an appropriate taxonomic key.
ACCLIMATION
- Acclimation period: 14 days
- Culturing media and conditions (same as test or not): The culture medium used for both the range-finding and definitive tests was the same as that used to maintain the stock culture.
- Any deformed or abnormal cells observed: The number of algal cells/ml in each test vessel and the occurrence of relative size differences, unusual cell shapes, colors, flocculations, adherence of cells to test containers, or aggregation of cells ' was determined visually by means of direct microscopic examination with a hemocytometer.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No applicable
Test conditions
- Hardness:
- No data available
- Test temperature:
- 23.5 - 23.9 deg. C
- pH:
- 7.0 - 7.5
- Dissolved oxygen:
- No data
- Salinity:
- Not applicable since used freshwater
- Nominal and measured concentrations:
- Nominal: 0.3, 3.3, 33, 330, and 3,300 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: The test vessels were 250 ml glass Erlenmeyer flasks that contained 100 ml of test solution (water depth was approximately 3 cm) and were capped with inverted glass beakers.
- Type: closed
- Material, size, headspace, fill volume: 250 ml glass conical flasks filled with 100 ml of test preparation
- Aeration: no data.
- Type of flow-through (e.g. peristaltic or proportional diluter): not applicable.
- Renewal rate of test solution (frequency/flow rate): not applicable.
- Initial cells density: 104 cells per ml.
- Control end cells density: 1.9x106 cells per ml.
- No. of organisms per vessel: no data.
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): not applicable.
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used: not applicable.
TEST MEDIUM / WATER PARAMETERS:
- Source/preparation of dilution water: water used for acclimation of test organisms and for all toxicity testing was sterile enriched media (U.S. EPA, 1978; T.R. Wilbury Standard Operating Procedure number 6) adjusted to a pH of 7.5 ± 0.1 prior to use with 0.1 N hydrochloric acid. Media used for the definitive toxicity test contained <10 mg/L particulate matter at the start of the toxicity test and 41 mg/L particulate matter at the end of the test (the final value resulted, at least in part, from the presence of algal cells).
OTHER TEST CONDITIONS
- Sterile test conditions: No
- Adjustment of pH: with 0.1N HCl.
- Photoperiod: continuous illumination
- Light intensity and quality: cool-white fluorescent lights that provided a light intensity approximately 400 footcandles.
- Salinity (for marine algae): not applicable.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: determined visually by means of direct microscopic examination with a hemocytometer.
- Chlorophyll measurement: No.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10x
- Justification for using less concentration than requested by guideline: not applicable.
- Range finding study: same as described above.
- Test concentrations: same as described above.
- Results used to determine the conditions for the definitive study: the study report was entitled as “range –finding study”, but it was a very comprehensive study and was regarded as final main study. Results are present in the following sections. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 27 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- not specified
- Remarks:
- cells/mL
- Basis for effect:
- cell number
- Remarks on result:
- other: 3.3 - 330 mg/L
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: specific growth rate
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: cell/mL
- Basis for effect:
- cell number
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: growth rate
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Unusual cell shape: There were no abnormalities detected in any of the control or test cultures
- Colour differences: no effects.
- Flocculation: no effects
- Adherence to test vessels: no effects.
- Aggregation of algal cells: no effects.
- Any stimulation of growth found in any treatment: no effects.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None.
- Effect concentrations exceeding solubility of substance in test medium: None. - Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- None
Any other information on results incl. tables
Table 1: Test Results After 96 Hours
Concentration (mg/L) |
Average cells /ml |
Percent of Control |
0 (control) |
1,901,000 |
─ |
0.3 |
1,875,000 |
99 |
3.3 |
1,825,000 |
96 |
33 |
831,000 |
44 |
330 |
42,000 |
2 |
3300 |
<10,000 |
<1 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure of freshwater alga to test material at concentrations of 0.3, 3.3, 33, 330, and 3,300 mg/L, plus an untreated control, was conducted in accordance with OECD Guideline 201 and US EPA (TSC) 797.1050. The study report was entitled as “range –finding study”, but the report was comprehensive and well documented. Therefore this study was regarded equivalent to definitive study with Klimisch code of 2.
From this study, a 96 hour EC50 of 27 mg/L (95% confidence interval = 3.3 to 330 mg/L) when calculated using cells/ml, and 120 mg/L (95% confidence interval =96 to 160 mg/L) when determined using the average specific growth rate were obtained. The 96 hour NOEC is 3.3 mg/L when determined using either the number of cells/ml or the average specific growth rate. - Executive summary:
The acute toxicity of the water accommodated fraction (WAF) of test material to the freshwater alga,Selenastrum capricornutum,was investigated during a range-finding study conducted at T.R. Wilbury Laboratories, Inc., in,. The test, which was designed to establish the approximate toxicity of the test substance, was performed for 96 hours from September 29 to October 3, 1997.
The test was performed at 24 ± 1°C under static conditions with a control (0 mg/L) and five concentrations of WAF (0.3, 3.3, 33, 330, and 3,300 mg/L). The dilution water was sterile enriched media adjusted to a pH of 7.5± 0.1. The five WAFS were prepared by formulating individual concentrations of the test substance and dilution water in mixing vessels equipped with a magnetic stirrer, stirring the mixtures for approximately 20 hours, settling the mixtures for approximately four hours, and siphoning the water phase containing the WAF. During the mixing period, the vortex extended approximately 25% of the distance from the water surface to the bottom of the mixing vessels.
The 3,300 mg/L test solution was cloudy throughout the test. No other insoluble material was noted during the test. Exposure of the algae to the test substance resulted in the following growth after 96 hours:
Concentration (mg/L)
Average cells /ml
Percent of Control
0 (control)
1,901,000
─
0.3
1,875,000
99
3.3
1,825,000
96
33
831,000
44
330
42,000
2
3300
<10,000
<1
Exposure of freshwater alga to the test item resulted in a 96 hour EC50 of 27 mg/L (95% confidence interval = 3.3 to 330 mg/L) when calculated using cells/ml, and 120 mg/L (95% confidence interval =96 to 160 mg/L) when determined using the average specific growth rate. The 96 hour NOEC is 3.3 mg/L when determined using either the number of cells/ml or the average specific growth rate.
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