Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline test, basic data given

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989
Reference Type:
secondary source
Title:
2,3,4-TRICHLORO-1-BUTENE
Author:
ECB
Year:
2000
Bibliographic source:
ECB IUCLID dataset
Reference Type:
secondary source
Title:
2,3,4-TRICHLORO-1-BUTENE - CAS No: 2431-50-7
Author:
OECD
Year:
1993
Bibliographic source:
Screening Information Data Set (SIDS) of OECD High Production Volume Chemicals Programme, (1993)

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Test Env. Federal Agency (UBA) 1984. Static test
Principles of method if other than guideline:
Daphnien-Schwimmunfaehigkeits-Test, UBA-Verfahrensvorschlag Mai 1984, Bestimmung der Schwimmunfaehigkeit beim Wasserfloh Daphnia magna, EC0, EC50, EC100 24h, static
GLP compliance:
yes
Remarks:
but no certificate available

Test material

Constituent 1
Chemical structure
Reference substance name:
2,3,4-trichlorobut-1-ene
EC Number:
219-397-9
EC Name:
2,3,4-trichlorobut-1-ene
Cas Number:
2431-50-7
Molecular formula:
C4H5Cl3
IUPAC Name:
2,3,4-trichlorobut-1-ene
Details on test material:
- Name of test material : 2,3,4-Trichlorbuten-1
- Analytical purity: 94% (cited in ECB IUCLUID)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the stock solution, 200 mg/L was weighed into water and stirred on a magnetic stirrer for three hours. The solution was kept in movement while drawing off the quantities necessary to produce the test concentrations.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Waterflea
- Strain: Daphnia magna Straus
- Source: Original from German health authority (BGA), Berlin, than breed in laboratory of Bayer AG, Leverkusen
- Age at study initiation : 6-24 hours

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Nominal and measured concentrations:
Nominal concentrations: Control, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 5.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: cylindrical beaker (diameter 4 cm, high: 6.5 cm), 20 ml test medium
- Aeration: no

- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered tapwater (gravel-pit Mohnheim)


EFFECT PARAMETERS MEASURED: mobility after 24 h
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
35 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
71 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
EC0=2.0 mg/L (potassium dichromate)

Applicant's summary and conclusion