L-97-034-PB

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23rd April - 7th August 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Pentaerythritol mixed esters with 2-methyl butyric acid, n-pentanoic acid, n-heptanoic acid, 3,5,5-trimethylhexanoic acid, n-octanoic acid, n-decanoic acid

Cas Number:

141686-49-9

IUPAC Name:

Pentaerythritol mixed esters with 2-methyl butyric acid, n-pentanoic acid, n-heptanoic acid, 3,5,5-trimethylhexanoic acid, n-octanoic acid, n-decanoic acid

Constituent 2

Reference substance name:

Trimethylol propane mixed esters with 2-methyl butyric acid, n-pentanoic acid, n-heptanoic acid, 3,5,5-trimethylhexanoic acid, n-octanoic acid, n-decanoic acid

Cas Number:

141686-50-2

IUPAC Name:

Trimethylol propane mixed esters with 2-methyl butyric acid, n-pentanoic acid, n-heptanoic acid, 3,5,5-trimethylhexanoic acid, n-octanoic acid, n-decanoic acid

Test material form:

liquid

Details on test material:

Identification: Hatcol ® 1772
Appearance/physical state: Clear colorless liquid
Purity: 100% (no solvent present)
Composition: 70% pentaerythritol esters described by CAS# 141686-49-9; 30% trimethylolpropane esters described by CAS# 141686-50-2

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST SPECIES
The test was carried out using juvenile rainbow trout (Oncorhynchus mykiss). Fish were obtained from Brow Well Fisheries Ltd, Phoenix Cottage, Hebden, Nr Skipton, UK and maintained in-house since 13 July 1998. Fish were maintained in a glass fibre tank with a 'single pass' water renewal system. Fish were acclimatised to test conditions from 22 July 1998 to 3 August 1998. The lighting cycle was controlled to give 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods.

The water temperature was controlled at 14°C with a dissolved oxygen content of greater than or equal to 9.3 mg O2/L. These parameters were recorded daily. The stock fish were fed commercial trout pellets which was discontinued approximately 26 hours prior to the start of the definitive study. Food was withheld for 26 hours as opposed to 48 hours. However, this was not considered to affect the results or validity of the study given that no mortalities or sub-lethal effects of exposure were observed in the control fish used in the study. There was less than 1% mortality in the 7 days prior to the start of the test and the fish had a mean standard length of 4.6 cm (sd = 0.4) and a mean weight of 1.36 g (sd = 0.29) at the end of the definitive study. Based on the mean weight value this gave a loading rate of 0.68 g bodyweight/litre.

The diet and diluent water are considered not to contain any contaminant that would affect the integrity and outcome of the study.

TEST WATER
The test water used for both the range-finding and definitive studies was the same as that used to maintain the stock fish.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

After 24, 48, 72 and 96 hours any mortalities or sub-lethal effects of exposure were determined by visual inspection of the test fish.

Test conditions

Test temperature:

13 ± 1°C

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LLR50 for the test material to rainbow trout (Oncorhynchus mykiss) based on nominal test concentrations was greater than 100 mg/l loading rate WAF. Correspondingly the No Observed Effect Concentration was equal 100 mg/l loading rate WAF.

Executive summary:

Introduction

A study was performed to assess the acute toxicity of the test material to rainbow trout (Oncorhynchus mykiss). The method followed that described in the OECD Guidelines for Testing of Chemicals (1992) No. 203 "Fish, Acute Toxicity Test," referenced as Method C1 of Commission Directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC); US CFR Title 40, Part 797, Section 1400, US EPA Pesticide Assessment Guidelines, Sub-Division E, Section 72 -2 and US EPA Draft Ecological Effects Test Guidelines OPPTS 850.1075.

Method

Following a preliminary range-finding study fish were exposed, in three groups of ten, to a Water Accommodated Fraction (WAF) of the test material, at a nominal loading rate of 100 mg/l for a period of 96 hours under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the study until termination after 96 hours.

Conclusion

The 96-hour LLR50 for the test material to rainbow trout (Oncorhynchus mykiss) based on nominal test concentrations was greater than 100 mg/l loading rate WAF. Correspondingly the No Observed Effect Concentration was equal 100 mg/l loading rate WAF.