Docosyl acrylate

Short-term toxicity to fish

Description of key information

In the short term toxicity test to fish the LC50 value of the test substance, 2-Propenoic acid, C16-18-alkyl esters (read across) based on the loading rate was determined to be >100 mg/L .

In an acute toxicity test to Zebrafish of the read across test substance Acrylate 22 45% (Behenylacrylate), the NOEL was laid down as 100 mg/L. The LL50 after 96 hours was > 100 mg/L. All effect levels are given based on the nominal level of the test item. Therefore the test substance was found to cause no toxic effects to Zebrafish after 96 hours when tested with a loading level of 100 mg/L.

Based on this result the LC50 value of 2-Propenoic acid, docosyl ester is also considered to be >100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The short term toxicity test to fish was conducted according to the OECD guideline 203.

The acute lethal toxicity of 2-Propenoic acid, C16-18-alkyl esters to Danio rerio (Zebrafish) (read across) was investigated under static exposure conditions over a period of 96 h. The singly applied loading rate was 100 mg/L.

GC-FID analysis of the test substance was conducted in the definitive test at the beginning of the test and after 24, 48, 72 and 96 h of incubation. The test concentration was below the quantification limit of 0.02 mg/L throughout the test. Thus, evaluation was based on the loading rate. At the loading rate of 100 mg/L, none of a total of seven fish died after 96 h of exposure. No mortality was observed in the blank control. Toxic effects other than mortality, e.g. loss of coordination, hypo- or hyperactivity and swimming on the back, were not observed. The no-observed-effect concentration (NOEC) was 100 mg/L with respect to the loading rate. 100% mortality (LC100) could not be detected.

Based on the binominal theory, the absence of mortality provides at least 99% confidence that the LC50 is greater than the applied loading rate of 100 mg/L.

Based on this result the LC50 value of 2-Propenoic acid, docosyl ester is also considered to be >100 mg/L.

The acute toxicity to fish (zebrafish), of the read across test substance Acrylate 22 45% (Behenylacrylate) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1992) and EU Council Regulation No. 440/2008/ C.1 (2008). A static test was performed. A single water accommodated fraction (WAF) of 100 mg/L was prepared and tested. Per definition of the WAF, all terms related to concentration Ievels were given as loading Ievels because partly dissolved compounds and mixtures cannot be related to concentrations. The duration of the testwas 96 hours. Seven test organisms each were exposed to the WAF and the control. Water quality parameters pH-value, temperature and oxygen-saturation, measured after 0, 24, 48, 72 and 96 h, were determined to be within the acceptable Iimits. The concentration of the test item in the loading Ievel and the control were analytically verified via analysis of organic carbon (TOC, according to DIN EN 1484) at the start of the exposure. All effect Ievels are given based on the nominal loading of the test item.

The NOEL was laid down as 100 mg/L. The LL50 after 96 hours was > 100 mg/L. All effect levels are given based on the nominal level of the test item. Therefore the test substance was found to cause no toxic effects to Zebrafish after 96 hours when tested with a loading level of 100 mg/L.