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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Three acute freshwater fish studies were assessed for this endpoint. Two limit tests and one actual determined LC50 value are available. The actual determined concrete LC50 value is used as key value and expressed as DTPA-Fe(NH4)2 amounts to 1063 mg/L.

One valid chronic study with the crimson-spotted rainbowfish was available for assessment. Based on a 28 -day exposure with the crimson spotted rainbowfish, the NOEC for DTPA-Fe(NH4)2 was 123 mg/Lbased on a lack of effects on reproduction. 

Five acute toxicity tests with aquatic invertebrates were assessed for this endpoint, and all studies were found to be of good quality and reliable for use in the risk assessment process. The 48-hour LC50 for Daphnia carinata, a freshwater species, was 245 mg/L DTPA acid, which is equivalent to 299 mg/L DTPA-Fe(NH4)2. The 96-hour LC50 for Crangon crangon, a saltwater species, was 6020 mg/L pentasodium DTPA, which is equivalent to 5744 mg/L DTPA-Fe(NH4)2.

Two chronic freshwater studies with DTPA (free acid) were conducted with Daphnia carinata. The 18-day NOEC for reproduction in Daphnia carinata was equivalent to 61 mg/L DTPA-Fe(NH4)2.

A well performed study with DTPA-FeNaH is available for algae. However, due to the photo-instability of the compound and coloration of test solutions, actual measured concentrations showed some unexpected results, making it difficult to conclude based on these geometric mean measured values. Still, based on actual and nominal concentrations the chronic ErC10 value was confirmed > 1 mg/L, indicating low hazard to the environment.

Additional well performed studies with EDTA-FeNa, without the indicated issues above, confirm the low toxicity to algae of these iron chelates, when micronutrient scavenging is taken into account in the experimental set-up.

If not, these substances possess properties indicating a (acute) hazard to algae. However, the effect is associated with the chelation of essential nutrients and is not considered a relevant environmental hazard in view of the abundance of such nutrients in the aquatic environment.


Taken the most reliable data together, the lowest chronic aquatic toxicity value for DTPA-Fe(NH4)2 is the 18 -day NOEC of 61 mg/L based on D. carinata reproduction. Daphnids are considered the most sensitive species and thus most relevant to consider for environmental risk assessment.