Decamethylenediamine

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011-12-05 to 2011-05-20

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study; restriction: result slightly outside range of applicability stated in guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

(2001)

Deviations:

yes

Remarks:

(result 5.2 slightly outside range 1.5 - 5.0)

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Version / remarks:

(2008)

Deviations:

yes

Remarks:

(result 5.2 slightly outside range 1.5 - 5.0)

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

no

Test temperature:

ambient (buffered test: 24.0 to 24.9 °C; unbuffered test: 23.8 to 24.8 °C)

Details on study design: HPLC method:

Apparatus: HPLC System HP 1100 (Agilent) with refractometer HP 1100 (Agilent)

HPLC conditions:
Stationary phase: Zorbax CN, 250 x 4 mm, 5 µm
Mobile phase (eluent):
- buffered testing:
methanol / buffer (pH 7.5) 65/35 (v/v)
(buffer: 16 ml 0.2 mol NaH2PO4 + 84 ml 0.2 mol Na2HPO4/ 1 l purified water; adjusted to pH 7.5 with H3PO4)
- dissociated testing:
methanol / purified water (pH 6.3) 65/35 (v/v)
Flow rate: 1.0 ml/min
Injection volume: 20 µl
Detection: refractive index

Urea was used as dead time marker.

Evaluation: external standard method

Reference compounds:

No. Reference sub. Purity (meth.) Weight / solvent
0 Urea 99.8 % (DSC) 25.52 mg / 25 ml eluent
1 Acetanilide 100 % (HPLC) 23.06 mg / 25 ml solvent 1
2 Monuron 99.9 % (HPLC) 20.50 mg / 25 ml solvent 1
3 Triapenthenol > 99 % 20.03 mg / 25 ml solvent 1
4 Linuron 99.7 % (HPLC) 21.52 mg / 25 ml solvent 1
5 Fenthion 96.2 % (HPLC) 22.22 mg / 25 ml solvent 1
6 Trifluralin 99.2 % (GC) 20.64 mg / 25 ml solvent 1
7 Phenanthrene 99.2 % (HPLC) 21.72 mg / 25 ml solvent 2
8 4,4’-DDT 99.2 % (GC) 20.17 mg/ 25 ml solvent 2; only used for unbuffered testing

Solvent 1: methanol / water (purified) 65/35 (v/v)
Solvent 2: 23 ml methanol + 2 ml purified water

Type:

Koc

Value:

125 893

Temp.:

25 °C

Remarks on result:

other: buffered at pH 7.5

Type:

log Koc

Value:

5.1

Temp.:

25

Remarks on result:

other: buffered at pH 7.5

Summary of the results of the HPLC measurements of the reference compounds and the test item (buffered testing):

 

Sample no.^1)	tR1 [min]	tR2 [min]	tR [min]	k’	log k’(x)	log KOC(y)
0	2.52	2.52	2.52	---	---	---
1	2.93	2.94	2.94	0.167	-0.777	1.25
2	3.30	3.30	3.30	0.310	-0.509	1.99
3	3.59	3.59	3.59	0.425	-0.372	2.37
4	4.02	4.01	4.02	0.595	-0.225	2.59
5	5.10	5.10	5.10	1.024	0.010	3.31
6	6.69	6.68	6.68	1.651	0.218	3.94
7	5.41	5.40	5.41	1.147	0.060	4.09
8	7.72	7.71	7.72	2.063	0.314	5.63
log Koc of test substance:
Test item	15.01			4.956	0.695	5.147
Test item		15.04		4.968	0.696	5.150
Mean value						5.1

 

 

¹⁾ For the names of the reference compounds see test conditions.

 

For the test evaluation the data of the calibration items 7 and 8 were not taken into account because of their poor correlation with the results of the other calibration items.

Summary of the results of the HPLC measurements of the reference compounds and the test item (unbuffered testing):

Sample no.^1)	tR1 [min]	tR2 [min]	tR [min]	k’	log k’(x)	log KOC(y)
0	2.52	2.52	2.52	---	---	---
1	2.94	2.94	2.94	0.167	-0.777	1.25
2	3.29	3.30	3.30	0.320	-0.509	1.99
3	3.61	3.62	3.62	0.437	-0.360	2.37
4	4.00	4.01	4.01	0.591	-0.228	2.59
5	5.07	5.09	5.08	1.016	0.007	3.31
6	6.62	6.65	6.64	1.635	0.214	3.94
7	5.38	5.37	5.38	1.135	0.055	4.09
log Koc of test substance:
Test item	-			-	-	-
Test item		-		-	-	-
Mean value						-

 

 

¹⁾ For the names of the reference compounds see test conditions.

For the test evaluation the data of the calibration item 7 were not taken into account because of their poor correlation with the results of the other calibration items.

   

The test item was not eluted under dissociated conditions. Therefore, log Koc could not be determined.

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient on soil and on sewage sludge (Koc) of decamethylenediamine was determined according to OECD guideline 121 [2001] and EU test method C.19 [2008]. The result was log Koc = 5.1 (Koc = 125893) at pH 7.5 and 24.0-24.9 °C, indicating "very high" sorption potential (Blume scale). The test substance was not eluted when tested without buffer.

Executive summary:

The adsorption coefficient on soil and on sewage sludge (Koc) of decamethylenediamine was determined according to OECD guideline 121 [2001] and EU test method C.19 [2008]. The result was log Koc = 5.1 (Koc = 125893) at pH 7.5 and 24.0-24.9 °C, indicating "very high" sorption potential (Blume scale). The test substance was not eluted when tested without buffer.

Description of key information

The adsorption coefficient on soil and on sewage sludge (Koc) of decamethylenediamine was determined according to OECD guideline 121 [2001] and EU test method C.19 [2008]. The result was log Koc = 5.1 (Koc = 125893) at pH 7.5 and 24.0-24.9 °C, indicating "very high" sorption potential (Blume scale). The test substance was not eluted when tested without buffer.

Key value for chemical safety assessment

Koc at 20 °C:

125 893

Additional information