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EC number: 231-868-0 | CAS number: 7772-99-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1988-10-14 to 1988-12-12
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP guideline study with acceptable restrictions Both the negative and positive control data appear to be high in comparison with up to date control data from other testing laboratories. No target organ toxicity by a change of the PCE/NCE ratio could be observed in any of the treatment groups. The fluctuation of the re-scoring results for one male animal showing significant increase in MN-PCE frequency might be indicative for less experienced staff scoring the cells.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- adopted 1983-05-26
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- tin(II) bis(methanesulfonate)
- IUPAC Name:
- tin(II) bis(methanesulfonate)
- Reference substance name:
- Tin(II) bis(methanesulfonate)
- EC Number:
- 401-640-7
- EC Name:
- Tin(II) bis(methanesulfonate)
- IUPAC Name:
- 401-640-7
- Reference substance name:
- 53408-94-9
- Cas Number:
- 53408-94-9
- IUPAC Name:
- 53408-94-9
- Test material form:
- other: crystalline powder
- Details on test material:
- - Name of test material (as cited in study report): STANNOUS METHANE SULPHONATE (12.11.87)(supplied by Toxicol Laboratories Limited)
- Physical state: off white-coloured crystalline powder
- Storage condition of test material: plastic flip-top jar, below -20°C in the dark
Constituent 1
Constituent 2
Constituent 3
Test animals
- Species:
- mouse
- Strain:
- other: CFLP
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Interfauna (UK) Limited, Wyton, Huntingdon, Cambridgeshire
- Age at study initiation (males and females): approximately five to eight weeks old
- Weight at study initiation (males and females): 22 - 28 g
- Assigned to test groups randomly: yes
- Fasting period: 3 -4 hours fast immediately before dosing and for approximately two hours after dosing
- Housing: housed in groups of up to five by sex in solid-floor polypropylene cages with sawdust bedding
- Diet (ad libitum, except during fasting period): rat and Mouse Expanded Diet No. 1 (Special Diet Services Limited, Witham Essex, U.K.)
- Water (ad libitum, except during fasting period): mains drinking water
- Acclimation period: minimum period of five days
ENVIRONMENTAL CONDITIONS
- Temperature: 19 - 21 °C
- Relative humidity: 50 - 58%
- Air exchanges: approximately 15 changes/hour
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: distilled water
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS:
Test material was freshly prepared as a solution at the appropriate concentration in the vehicle after grinding to a fine powder using a mortar and pestle. - Duration of treatment / exposure:
- one treatment only
- Frequency of treatment:
- once
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1000 mg/kg
Basis:
actual ingested
- No. of animals per sex per dose:
- treatment group: 5 males / 5 females (total: three groups)
vehicle control group: 5 males / 5 females (total: three groups)
positive control group: 5 males / 5 females (total: one group) - Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide
- Doses / concentrations: 50 mg/kg
Examinations
- Tissues and cell types examined:
- Stained bone marrow smears were examined at random using light microscopy at x 1000 magnification. The incidence of micronucleated cells per 1000 polychromatic erythrocytes PCE per animal was scored. In addition, the number of normochromatic erythrocytes NCE associated with 1000 polychromatic erythrocytes was counted; these cells were also scored for incidence of micronuclei.
The ratio of normochromatic to polychromatic erythrocytes was calculated together with appropriate group mean values for males and females separately and combined. - Details of tissue and slide preparation:
- RANGE-FINDING TOXICITY STUDY:
A range-finding study was performed. Groups of mice (2 males / 2 females) were dosed with stannous methane sulphonate (12.11.87) as follows: 500, 1000, 2000, and 5000 mg/kg.
All animals were dosed once only at the appropriate dose level by gavage. The volume administered to each animal was calculated according to its fasted bodyweight at the time of dosing.
Animals were observed 1 and 4 hours after dosing and subsequently once daily for 3 days. Deaths and evidence of overt toxicity were recorded at each observation. No necropsies were performed. At 72-hours after dosing, up to 2 mice of each sex were sampled for bone-marrow cells to evaluate any specific bone-marrow toxicity in the absence of deaths.
TREATMENT AND SAMPLING TIMES:
Three groups, each of five males and five female mice were dosed once only with test material at the "maximum tolerated dose level". One group of mice was killed by cervical dislocation 24 hours following treatment, a second at 48 hours and a third at 72 hours. In addition, three groups (5 males / 5 females each) were treated with the vehicle alone and one group (5 males / 5 females) was treated with the positive control. The vehicle control groups were killed 24, 48 and 72 hours following treatment and positive control group animals were killed 24 hours following treatment.
DETAILS OF SLIDE PREPARATION:
Immediately following sacrifice, one femur was dissected from each animal, aspirated with foetal calf serum and bone marrow smears prepared following centrifugation and re-suspension. The smears were air-dried, fixed in absolute methanol, and stained in May-Grünwald/Giemsa.
OTHER:
All animals were observed for signs of overt toxicity and death 1 and 4 hours after dosing and then once daily as applicable. - Evaluation criteria:
- A comparison was made between the number of micronucleated polychromatic erythrocytes occurring in each of the three test material groups and the number occurring in the corresponding vehicle control groups.
A positive mutagenic response is demonstrated when a statistically significant increase in the number of micronucleated polychromatic erythrocytes is observed for either the 24, 48 or 72 hour kill times.
If the above criteria are not demonstrated, then the test material is considered to be non-mutagenic under the conditions of the test.
A positive response for bone marrow toxicity is demonstrated when the treatment group mean normochromatic to polychromatic ratio is twice the vehicle control value or when a treatment related increase is shown to be statistically significant. - Statistics:
- If necessary, and where possible, all data were statistically analysed using the Mann-Whitney U-Test.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Remarks:
- please refer to the field "Additional information on results" below
- Toxicity:
- yes
- Remarks:
- please refer to the field "Additional information on results" below
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not examined
- Positive controls validity:
- not specified
- Additional information on results:
- RESULTS OF RANGE-FINDING TOXICITY STUDY
- 5000 mg/kg dose level: all four animals showed adverse clinical symptoms which included: hunched posture, lethargy, pilo-erection, decreased respiratory rate and ptosis. Three of these four animals subsequently died on the first day post treatment while the fourth animal, a male, survived for the full period of the test showing fewer clinical signs on successive days.
- 2000 mg/kg dose level: the four animals showed similar clinical symptoms to those treated with 5000 mg/kg. Two of the four animals died, one on day 0 and the other on day two.
- 500 and 1000 mg/kg dose levels: all animals exhibited hunched posture, lethargy, pilo-erection and decreased respiratory rate one hour after dosing. At four hours post dosing the respiration rate was back to normal and on days 1, 2 and 3 post dosing no clinical symptoms were observed. No evidence of excessive bone marrow toxicity was observed.
The 1000 mg/kg dose level was selected as the maximum tolerated dose used for the micronucleus study.
RESULTS OF DEFINITIVE STUDY
- mortality and clinical observations: one of the animals in the 48-hour treatment group died on the day of dosing. The clinical symptoms observed were similar to those seen in the toxicity range-finding study, although occasionally animals showed additional symptoms of ataxia and body tremors.
- there were no significant increases in the frequency of micronucleated PCE's in any of the treatment groups.
- one male in the 24-hour treatment group had a frequency of 13 micronucleated PCE#s per 1000 PCE's. The slide from this animal was rescored independently 3 times and gave scores of 6, 9 and 8 micronucleated PCE's per 1000 PCE's . According to the authors, it may be concluded that this animal did have a higher than normal frequency of micronucleated PCE's although it was probably not treatment-related.
- there were no significant increases in the frequency of micronucleated NCE's in the treatment groups and also there was no significant changes in the NCE/PCE ratio.
- positive control group: a marked increase in the incidence of micronucleated polychromatic erythrocytes was observed.
- the test material did not induce a statistically significant increase in micronucleated PCE'S. Stannous methane sulphonate (12.11.87) was considered therefore to be non-mutagenic under the conditions of the test.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Stannous methane sulphonate was found not to produce micronuclei in polychromatic erythrocytes of mice under the conditions of the test.
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