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EC number: 200-238-7 | CAS number: 55-56-1
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The test was conducted using Chlorhexidine digluconate as test substance. As Chlorhexidine base is the active component, the results can be converted to equivalents of CHD-Base.
The test on reproduction of Daphnia magna was performed according to the OECD Guideline 211. Monitoring of the Chlorhexidine digluconate concentration revealed that the stability of the test substance concentration was > 80% of nominal. Test parameters were mortality of parent daphnia, reproduction, first brood release, intrinsic rates of natural increase, appearance of neonates, condition of parent daphnia, total length and body weight of parent daphnia.
A steep dose-response relationship was found. While at 0.012 mg CHD-Base/L no significant effects were observed, all parent animals died at 0.034 mg CHD-Base/L. The NOEC was found to be 0.012 mg CHD-Base/L for all examined endpoints.
This result demonstrates that Chlorhexidine does not exert any chronic toxicity in D. magna and that solely an acute mode of action can be assumed. This conclusion is in full accordance with the acute D. magna EC50 of 0.049 mg CHD-Base/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.012 mg/L
Additional information
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