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Short-term toxicity to aquatic invertebrates

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Description of key information

Considering that the lower 48 hour EC50 value of 0.9 mg/L obtained from an acute daphnia toxicity study with amides, C16-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl) was attributed to the presence of an impurity, the more reliable acute 48 hour EC50 value in freshwater invertebrates of 3.2 mg/L conducted with structurally similar amides, C8 -18 and C18 -unsatd., N,N-bis(hydroxyethyl) was considered more appropriate for hazard assessment of amides, C16-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
3.2 mg/L

Additional information

A study was conducted to determine the acute toxicity of amides, C16-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl) toDaphnia magnain a static system. Juvenile water fleas were exposed for 48 hours to the test substance at 0, 0.5, 0.7, 1.0, 1.4, 2.0, 2.8, 4.0, 5.6 and 8.0 mg/L. Immobility was recorded at 24 and 48 hours. Under the study conditions, the 48 hour EC50of the test substance forDaphnia magnawas 0.9 mg/L (nominal) due to the presence of an impurity,. The 48 hour NOEC was 0.5 mg/L (nominal) (Scholz, 1991).

An acute toxicity study according to OECD guideline 202 was performed to evaluate the immobilisation potential of structurally similar amides, C8-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl) inDaphnia magna. The test animals in duplicate petri dishes were exposed to the test substance at 0, 1, 2, 4, 8 and 16 mg/L under static conditions. Observations were performed at 0, 24 and 48 hours to determine the % immobilised daphnids. Under the test conditions, the 48 hour EC50value was calculated to be 3.2 mg/L. The LOEC and the NOEC values at 48 hour were 2.0 and 1.0 mg/L, respectively (Olsson T, 1994).

An OECD guideline 202 study with deviations was conducted to evaluate the acute toxicity of structurally similar amides, C12-18(even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl) toDaphnia magnaunder static conditions.Daphnia magna(20 per dose) were exposed to the test substance in concentration ranges of 1 -11 mg/L for 24 hours and immobilisation was determined. Based on the results, the 24 hour EC0, EC50and EC100were 2.0, 3.3 and 5.6 mg/L, respectively (Gode, 2001b).