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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

A well conducted study of structurally similar amides, C8-18 and C18-unsatd., N,N-bis(hydroxyethyl) conducted in Pseudomonas putida (bacteria) resulted in EC10 and EC50 values of 0.83 and 6 g/L respectively. Inhibition of cell growth was observed at 1.0 mg/L. The same data will be used by read across for amides, C12, N, N-bis(2-hydroxyethyl).

Key value for chemical safety assessment

EC50 for microorganisms:
6 g/L
EC10 or NOEC for microorganisms:
0.83 g/L

Additional information

A study was conducted to assess the toxicity of structurally similar amides, C8 -18 and C18 -unsatd., N,N-bis(hydroxyethyl) toPseudomona putidaaccording to DIN guideline 38412 L8.Pseudomonas putidacolonies, in triplicate, were exposed to the test substance at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 72 hours. The EC10was 0.83 g/L and the EC506.0 g/L. Inhibition of cell growth was observed as of 1.0 g/L.