Methyl vinyl ether

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to Guideline study with acceptable restrictions (report not available for the submitter, no further details available). Presumably rapid evaporation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methyl vinyl ether

EC Number:

203-475-4

EC Name:

Methyl vinyl ether

Cas Number:

107-25-5

Molecular formula:

C3H6O

IUPAC Name:

methoxyethene

Details on test material:

methyl vinyl ether (MVE)
no further details

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

Animals were kept in rooms designed to maintain adequate environmental conditions, and were given a commercial diet and municipal water ad libitum.

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: unexposed eyes

Amount / concentration applied:

undiluted
Amount applied: 0.1 mL

Duration of treatment / exposure:

unwashed

Observation period (in vivo):

scoring days 1, 2, 3, 7, 14, and 21

Number of animals or in vitro replicates:

6 male and female New Zealand White Rabbits were used for the study

Details on study design:

The dose (0.1 mL of undiluted, cold test material) was instilled into the conjunctival sac of one eye per animal, and eyelids were held together for one second. The eyes were scored based on a defined scale (evaluating the cornea, iris, and conjunctivae) at days 1, 2, 3, 7, 14, and 21. Fluorescein staining was used to determine corneal injury before dosing and at day 1 readings.

Results and discussion

In vivo

Results

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 24 h

Score:

1

Max. score:

3

Reversibility:

fully reversible within: 24 h

Remarks on result:

other: No corneal injury or iritis; minor transient conjunctival irritation in 5/6 rabbits

Irritant / corrosive response data:

No corneal injury or iritis in any of 6 eyes was noted. Minor transient conjunctival irritation in 5/6 rabbits from 0.1 mL undiluted liquid was observed, but resolved within 24 hours. No further details available.
One animal was sacrificed at 4 days after it developed moribund appearance, which was probably not related to treatment.

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information

Conclusions:

The instillation of 0.1 mL undiluted, cold liquid resulted only in minor transient conjunctival irritation in 5/6 New Zealand White rabbits; the effects were fully reversible within 24 h.