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EC number: 203-475-4 | CAS number: 107-25-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Preguideline study. Similar to GLP guideline study. Purity of TS not reported; not tested in TA102 or E.coli WP2.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Principles of method if other than guideline:
- adopted 1983
- GLP compliance:
- yes
- Remarks:
- (Litton Bionetics, Inc.; quality control comparable to GLP)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl vinyl ether
- EC Number:
- 203-475-4
- EC Name:
- Methyl vinyl ether
- Cas Number:
- 107-25-5
- Molecular formula:
- C3H6O
- IUPAC Name:
- methoxyethene
- Details on test material:
- Methyl vinyl ether (MVE)
no information on
purity reported
Constituent 1
Method
- Target gene:
- His-
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix; S-9 fraction from Arochlor 1254-induced male Sprague-Dawley rat liver was used
- Test concentrations with justification for top dose:
- vapour concentration:
0, 1, 5, 10, 25% 1st trial
0, 0.5, 1, 2.5, 5, 10% 2nd trial - Vehicle / solvent:
- no vehicle, exposure to vapour
Controls
- Untreated negative controls:
- yes
- Remarks:
- (no solvent used; exposure to vapour)
- Positive controls:
- yes
- Positive control substance:
- other: Without metabolic activation: sodium azide (1 µg/plate; TA1535, TA100); 2-nitrofluorene (10 µg/plate TA1538, TA98); 9-aminoacridine (50 µg/plate; TA1537); With metabolic activation: 2-anthramine (2.5 µg/plate for all strains)
- Details on test system and experimental conditions:
- Standard plate test with and without metabolic activation using a gas-tight incubation chamber. Tests were run in duplicate. Plates with the test organisms were placed into a gas-tight incubation chamber. A flowmeter was used to introduce known amounts of gas from yielding atmosphere MVE concentrations of 0.5 to 25%. The chamber containing the plates was incubated at 37°C for 2 days.
Second trial with TA98, TA100 and TA1535 - Evaluation criteria:
- Strains TA-1535, TA-1537 and TA-1538
If the solvent control value is within the normal range, a test material that produces a positive dose response over three concentrations with the highest increase equal to three times the solvent control value will be considered to be mutagenic.
Strains TA-98 and TA-100
If the solvent control value is within the normal range, a test material that produces a positive dose response over three concentrations with the highest increase equal to twice the solvent control value for TA-98 and TA-100 will be considered to be mutagenic.
Reproducibility. - Statistics:
- no
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA1535 TA1537 TA1538 TA98 TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (at a vapour concentration of 25%)
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- MVE did not increase the number of revertants in any tester strain at 1, 5, 10, or 25% with or without metabolic activation. The substance was cytotoxic at 25% with all strains; in TA98 and TA1535 also at 10% vapour concentration in the 2nd trial. Negative and positive control plates gave results as expected. Repeat tests with and without activation with test strains TA 1535 and TA100 at concentrations of 0.5, 1, 2, 5, and 10% were also negative. TA98 was also included in this series because of unacceptable control values and gave equally negative results.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Revertants in the Ames test after exposure to MVE vapour
Vapour concentration |
TA1535 |
TA1537 |
TA1538 |
TA98 |
TA100 |
|||||
Plate1 |
Plate2 |
Plate1 |
Plate2 |
Plate1 |
Plate2 |
Plate1 |
Plate2 |
Plate1 |
Plate2 |
|
Without metabolic activation, 1st trial |
||||||||||
Neg. control |
20 |
16 |
11 |
7 |
21 |
17 |
130 |
151 |
274 |
249 |
Pos. control |
1045 |
939 |
279 |
422 |
929 |
1137 |
602 |
1289 |
1186 |
1149 |
1% |
19 |
29 |
4 |
3 |
5 |
14 |
109 |
93 |
229 |
C |
5% |
13 |
9 |
6 |
6 |
8 |
11 |
82 |
100 |
205 |
265 |
10% |
14 |
18 |
9 |
4 |
8 |
7 |
94 |
95 |
211 |
217 |
25% |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
With metabolic activation, 1st trial |
||||||||||
Neg. control |
5 |
10 |
18 |
20 |
18 |
21 |
123 |
121 |
257 |
268 |
Pos. control |
468 |
434 |
360 |
478 |
1918 |
2084 |
2174 |
1161 |
2170 |
2220 |
1% |
3 |
7 |
5 |
11 |
12 |
16 |
109 |
148 |
C |
C |
5% |
12 |
11 |
10 |
9 |
12 |
15 |
119 |
C |
252 |
C |
10% |
5 |
9 |
7 |
6 |
12 |
14 |
94 |
114 |
249 |
C |
25% |
1 |
0 |
0 |
0 |
1 |
1 |
0 |
0 |
0 |
0 |
Without metabolic activation, 2nd trial |
||||||||||
Neg. control |
10 |
14 |
No data |
No data |
28 |
27 |
162 |
154 |
||
Pos. control |
829 |
944 |
954 |
922 |
938 |
977 |
||||
0.5% |
15 |
9 |
Nd |
Nd |
150 |
170 |
||||
1% |
13 |
10 |
22 |
30 |
189 |
177 |
||||
2.5% |
9 |
14 |
Nd |
Nd |
189 |
209 |
||||
5% |
3 |
9 |
10 |
11 |
216 |
192 |
||||
10% |
0 |
0 |
5 |
6 |
104 |
98 |
||||
With metabolic activation, 2nd trial |
||||||||||
Neg. control |
16 |
10 |
34 |
23 |
199 |
179 |
||||
Pos. control |
379 |
382 |
1273 |
1075 |
991 |
902 |
||||
0.5% |
16 |
21 |
Nd |
Nd |
275 |
200 |
||||
1% |
20 |
11 |
31 |
21 |
183 |
206 |
||||
2% |
9 |
8 |
Nd |
Nd |
290 |
296 |
||||
5% |
9 |
12 |
16 |
19 |
234 |
251 |
||||
10% |
0 |
0 |
2 |
4 |
45 |
87 |
C: contaminated; Nd: no data
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Methyl vinyl ether was not mutagenic in S. typhimurium TA98, TA100, TA1535, TA1537, TA1538 in the presence or absence of metabolic activation
at vapour concentrations from 0.5 to 25%. Atmospheres containing
25% of the test substance were cytotoxic.
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