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EC number: 220-713-2 | CAS number: 2873-97-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian cell study: DNA damage and/or repair
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study with GLP and equivalent to OECD guideline 486 that was not available at the time of performance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 486 (Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo)
- Deviations:
- no
- Remarks:
- : no relevant deviations from the guideline that appeared 6 years later
- Principles of method if other than guideline:
- This test provides a method for investigating genotoxic effects of chemicals in liver cells. The end-point measured is indicative of DNA damage and subsequent repair. The liver is usually the major site of metabolism of absorbed compounds. It is thus an appropriate site to measure DNA damage in vivo. The UDS test with mammalian liver cells in vivo indicates DNA repair synthesis after excision and removal of a stretch of DNA containing a region of damage induced by chemical substances. The test is based on the incorporation of tritium labelled thymidine (3H-TdR) into the DNA of liver cells which have a low frequency of cells in the S-phase of the cell cycle. The uptake of 3H-TdR is determined by autoradiography.
- GLP compliance:
- yes
- Type of assay:
- unscheduled DNA synthesis
Test material
- Reference substance name:
- diacetone acrylamide
- IUPAC Name:
- diacetone acrylamide
- Details on test material:
- Appearance: White solid. Batch No.: A-46318Conditions of storage: room temperatureStability in aqueous solution: 10 hPurity: 99.2 %
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS- Source: Savo-Ivanovas, 7964 Kisslegg, Germany- Weight at study initiation: 160 - 180 g- Assigned to test groups randomly: yes- Housing: single, Makrolon, type I- Diet (e.g. ad libitum): pelleted Altromin 1324The animals were starved overnight (4 h treatment) or ca. 6 h (16 h treatment) before dosing.- Water ad libitum: tap water- Acclimation period: >= 5 dENVIRONMENTAL CONDITIONS- Temperature (°C): 21 +- 3- Humidity (%): 30 - 70- Photoperiod (hrs dark / hrs light): light from 6:00 - 18:00
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- Aqua bidest.
- Duration of treatment / exposure:
- 4 h and 16 h
- Frequency of treatment:
- Once
- Post exposure period:
- Rats were sacrificed after the exposure, the liver was perfused. Hepatocyte cultures were prepared and exposed to 3H-TdR for 4 h.
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:1000 mg/kg bwBasis:nominal conc.for the 4 h treatment period
- Remarks:
- Doses / Concentrations:1000 mg/kg bwBasis:nominal conc.for the high dose in the 16 h treatment period
- Remarks:
- Doses / Concentrations:100 mg/kg bwBasis:nominal conc.for the low dose in the 16 h treatment period
- No. of animals per sex per dose:
- 5 male rats per group.5 hepatocyte cultures were established for each rat.3 of the cultures from each animal were used for the UDS assay.
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- 2-acetylaminofluorene; dissolved in DMSO/polyethylene glycol 400 (1 + 9)Route of administration: orallyDoses / concentrations: 100 mg/kg bw
Examinations
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:Based on a range finding study at 1000 mg/kg bw. Result: no sign of toxicity.DETAILS on culture and slide PREPARATION:The washed hepatocytes were centrifuged and transferred into Williams medium E. Aliquots of freshly isolated hepatocytes in complete culture medium were added to 35 mm six-well cluster dishes. After an attachment period of ca. 1.5 h the medium was discarded. 3H-TdR was added for 4 h. After washing again the cultures were incubated overnight, followed by hypotonic treatment. The cells on coverslips were fixed and air dried. Autoradiographic processing:The cover slips were coated with Ilford K-2 photographic emulsion in the dark for 12 - 14 d at 4 °C.METHOD OF ANALYSIS:OTHER:
- Evaluation criteria:
- Evaluation was performed microscopically on coded slides. The number of silver grains above the nucleus was counted automatically. In addition, the number of grain counts of one nuclear-sized cytoplasm adjacent to the nucleus was counted. At least 2 slides per rat and 50 cells / slide were evaluated.A test substance is classified as positive if it induces either a statistically significant dose-related increase in radiolabel incorporation expressed as grains per nucleus or a reproducible and significant positive response for at least one of the test points.
- Statistics:
- Significance was evaluated by means of the non-parametric Mann-Whitney test.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- No toxic reaction occurred in any group. Viability and intro attachment of the hepatocytes was not affected to a relevant degree by the pre-treatment with the test substance.Neither the nuclear grains nor the resulting net grains were enhanced due to the in vivo treatment of the rats with the test substance for 4 h or 16 h.The positive control substance induced a distinct increase of the DNA repair.For the grain counts, see the attachment.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negativeThe test substance did not induce DNA-damage leading to repair synthesis.
- Executive summary:
The test substance was assessed in an in vivo / in vitro UDS assay, to detect a possible induced DNA repair in hepatocytes. The treatment periods were 4 h and 16 h. The highest dose level was 1000 mg / kg body weight. DNA repair was followed by autoradiography after incorporation of tritiated thymidine.
No toxic reaction occurred in any group. Viability and intro attachment of the hepatocytes was not affected to a relevant degree by the pre-treatment with the test substance.
Neither the nuclear grains nor the resulting net grains were enhanced due to the in vivo treatment of the rats with the test substance for 4 h or 16 h. The test substance did not induce DNA-damage leading to repair synthesis.
The positive control substance induced a distinct increase of the DNA repair.
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