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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Similar to Guideline Study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1981
Report Date:
1981

Materials and methods

Principles of method if other than guideline:
Method:according to OECD-Guideline 471
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Butin2-diol.1,4
- Analytical purity: > 99%

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium (TA 98, TA 100, TA 1535, TA 1537, TA 1538)
Metabolic activation:
with and without
Metabolic activation system:
S-9 mix(rat liver)
Test concentrations with justification for top dose:
20 - 5000 ug/plate

Results and discussion

Test results
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Remarks on result:
other: other: Salmonella typhimurium (TA 98, TA 100, TA 1535, TA 1537, TA 1538)
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Toxicity:

No bacteriotoxic effect was observed.

Mutagenicity:

An increase in the number of his+ revertants was not observed either without S-9 mix or after the addition of a metabolizing system.

According to the result ot the present study, the test substance Butin-2-diol-1,4 is not mutagenic in the Ames test under the experimental condition chosen here.

Applicant's summary and conclusion